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Team:Davidson-Missouri Western/Las
From 2008.igem.org
Las/Rhl cell signaling system
Responsible: Robert Cool, Alicia Allen, and Erin Feeney
Las System
Signal Molecule: An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)
Bacterial species: Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)
Receiver protein: LasR
Effect of binding: TXN activation of virulence genes, lasA, lasB, apr, toxR
Synthase: LasI enzyme
Target Genes: lasI, lasA, lasB, apr, toxR
Regulation: unknown
References
"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"
JP Pearson, EC Pesci and BH Iglewski [http://jb.asm.org/cgi/reprint/179/18/5756?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=las&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT]
"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"
Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci [http://jb.asm.org/cgi/content/full/187/13/4372?view=long&pmid=15968046 2]
Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa
Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden [http://jb.asm.org/cgi/content/full/185/12/3558?view=long&pmid=12775693 3]
Pending: [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1447470]
Rhl System
Signal Molecule: An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)
Bacterial species: Pseudomonas aeruginosa, gram(-)
Receiver Protein: Rhl R
Effect of Binding: activation of Rhamnosyl Transferase, then making RL (rhamnolipid)
Synthase: RhlA and RhlB
Target Genes: pqsABCDE and phnAB
Regulation: unknown
References
[http://jb.asm.org/cgi/reprint/189/13/4827 background information on Las and Rhl]
Parts Needed:
LasR + pro/term
RhlR + pro/term
RhlI + pro/term
pqsABCDE + pro/term
pqsR
pqsH + pro/term
phnAB
LasI
RBS
