Team:ESBS-Strasbourg/16 July 2008

From 2008.igem.org

< back

Contents

DryLab

michi:

  • IRESes s*** let's mutate our promoter
  • last chance for IRES: wrote email to Pospisek
  • Calculated size of our constructs: 6 kb (too big for vector?)

Wiki

  • Further content in all categories (MM)

Modeling

WetLab

Sandra:

  • Gel of the PCR from yesterday
  • Transformations
    • cells | part/vector | protocol 
 dh5a (Mariel) | pxj (10 ng)      | HeatShock (Maria)
       =       |      =           | KCM (Barbara)
       =       | LexA-BD (1 µl)   |      =
       =       | BB_pSB (2 µl)    |      =
       =       | BB_1763004 (2 µl)|      =
 dh5a (ours)   | pxj (10 ng)      | HS
 Top10         |     =            | =
 db3.1         |     =            | =
   =           | BB_p1010 (2 µl)  | =


Mariel repeats the PCR from Paul from yesterday with 10ng plasmids
=>Amplification of Gal4 succeed

Paul:

  • Purification of Gal4 PCR product, stored at -20°C


Katja:

  • Preparation of TENS buffer (not steril filtered)
  • Preparation of 3M NaAcO pH5
  • Miniprep with b.1) Protocol of the BBa_J63002(?) Plasmid to verify the identity by digestion after PCR failed

General

Our account is open again.(KK)

Quote of the day

Retrieved from "http://2008.igem.org/Team:ESBS-Strasbourg/16_July_2008"