Team:Hawaii/Meeting/2008-09- 8

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Contents

Agenda

  1. Exchange Lab Techniques & Protocols
    1. plasmid prep using low media volume, big flask (Margaret)
    2. SAP treatment of vector to prevent self-ligation, success? (Grace/Krystle)
    3. Gel cutting using SYBRSafe+Blue Light, [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Project:Phytoleum/Experiment/phaE_T855C_SigE_G855C_BioBricking_Site_Directed_Mutagenesis#Track_Yield_Loss_from_PCR.2C_post_ExoSAP.2FRestriction_Digestion.2C_and_post_Gel_Purification_Kit Yield Loss at each step](NW)
    4. 70min [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Phosphorylation_and_Hybridization_of_Oligonucleotide_Insert PNK+Oligo Annealing Protocol] (NW)
  2. Updates of progress from the past two weeks
    1. Grace
      1. Re-replacement of BB-pRL1383a MCS
    2. Krystle
      1. GFPf w/ tt? GFP w/ tt?
    3. Margaret
      1. Rep, OriT, OriV, etc...
      2. aadA, Omega
      3. P1 Lytic
    4. Norman
      1. Bioplastic synthase operon (phaABCE) construction progress
      2. site directed mutagenesis of two genes phaE and "sigma master switch"
  3. Logistics
    1. Travel itinerary (NW)
    2. Sign Waiver (All)

Minutes

Present: <Person A>, <Person B>

Action Items

  • <Person A>: Task

Coming Up


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