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Team:KULeuven/14 September 2008
From 2008.igem.org
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Lab Work
Wet Lab
We occupied ourself in the lab with the usual stuff:
- Miniprep: K145257.
- Electroporation: C0062+B0015, I712074+J23078 and K145275.
- Digests: K145257 was cut with EcoRI and XbaI, yesterday's digests were continued: cut with Pst I -> J23109, K145150+B0034 and K145013.cut with EcoR I -> K145272, K145013+B0015, K145001+B0015, K145254 and K145201.
- PCR to test electroporations of yesterday: We had colonies of J23116+B0034 and the PCR test showed that it was ligated properly.
- Ligation mix: K145014+pSB1A2, J23109+J23078, K145253+K145254, J23116+K145201, J23116+B0032+K145201 and (K145150+B0034)+C0062.
