Team:Paris/September 10

From 2008.igem.org

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Checking our ligases (following)

Analysis of the ligation transformants

Several colonies for the pUC19 transformation control
No colonies for the ligations

Analysis of the ligation products by electrophoresis

well n°

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

sample

1 kb
DNA ladder

undigested DNA
MP101.1

ligation products

100 bp
DNA ladder

temperature of ligation

16°C

4°C

ligase n°

L1

L2

L1

L2

duration of ligation

2 h

O/N

2 h

O/N

amount of ligase (units)

400 U

2 U

400 U

2 U

400 U

2 U

400 U

2 U

400 U

2 U

400 U

2 U

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