Michał K.
- PCR - translational fusion: AID + T7 RNA-polymerase
Primers:
Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translational fusion
Annealing temperature : 73°C
Elongation time: 4 minutes
20 cycles
- Gel electrophoresis and isolation of proper band (3300 bp). Fig. 1.
- Electrophoresis to estimate the concentration of isolated DNA.
Fig. 1. 1-DNA ladder; 2-PCR product-translational fusion
Rifampicin test #1
Michał L., Ewa, Marcin
Overnight culture of the following strains:
Top10
Top10 + 0.5 mM IPTG
and Top10 carrying:
pMPM-T5-AID + Glc
pTrc99a-AID + Glc
pMPM-T5-AID + 0.01% L-Ara
pTrc99a-AID + 0.5 mM IPTG
"
