From 2008.igem.org
pBca1256-K112400
Biobricking of promoter for grpE protein
PCR grpE-F and grpE-R on MG1655 (129 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L)
Product is pBca1256-K112400
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cb0001-grpE-F Forward Biobricking cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
cb0002-grpE-R Reverse Biobricking cgtagGGATCCcgcattcatctcgctaacttc
pBca1256-K112401
Biobricking of promoter for recA protein
PCR recA-F and recA-R on MG1655 (317 bp, EcoR1/BamH1)
Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L)
Product is pBca1256-K112401
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cb0003-recA-F Forward Biobricking cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
cb0004-recA-R Reverse Biobricking cgtagGGATCCcccagtgctgccgccaacgc
pBca1256-K112402
Biobricking of promoter for fabA protein
PCR fabA-F and fabA-R on MG1655 (285 bp, EcoR1/BamH1)
Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L)
Product is pBca1256-K112402
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cb0005-fabA-F Forward Biobricking cgataGAATTCatgAGATCTggccattacgttggctgaac
cb0006-fabA-R Reverse Biobricking cgtagGGATCCgaagttaccacccgtttcgg
pBca1256-K112403
Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase)
PCR cb10007-F and cb10008 on MG1655 (114 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L)
Product is pBca1256-K112403
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cb10007-phoA-F cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
cb10008-phoA-R cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112404
Biobricking of signal peptide (prepro) of lamB protein
PCR cb10009-F and cb10010 on MG1655 (132 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L)
Product is pBca1256-K112404
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cb10009-lamB-F cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
cb10010-lamB-R cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112405
Biobricking of promoter for cadAB genes
PCR cad-F and cad-R on MG1655 (402 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L)
Product is pBca1256-K112405
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cb10011-cad-F cgataGAATTCatgAGATCTggtatattccagacttctgttcc
cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
pBca1256-K112406
Biobricking of cadC promoter and cadC gene
PCR cadC-F and cadC-R on MG1655 (2336 bp, EcoR1/BamH1)
Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L)
Product is pBca1256-K112406
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cb10013-cadC-F cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc
cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
pBca1256-K112407
Biobricking of ygeF pseudogene promoter
PCR ygeF-F and ygeF-R on MG1655 (540 bp, EcoR1/BamH1)
Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L)
Product is pBca1256-K112407
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cb10014-ygeF-F cgataGAATTCatgAGATCTcagtttactcagtgaatcaatagagg
cb10015-ygeF-R cgtagGGATCCatttgattagagcaaaatccCaattcgcagc
pBjh1601CA-K112408
T4 derived lysis device
Sub into pBjh1601KA (5025 bp, EcoR1/BamH1)
Product is pBjh1601KA-K112408 (EcoR1/BamH1 3196/1824 S)
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