User:Jec105

In order to achieve our specifications of design previously described, we require the following constructs;

• Light sensing device - Converting a light input into an output of PoPS,
• Biomaterial production device- Converting a PoPS input into an output of biomaterial,
• Motility Control device - Converting a PoPS input into an output of motility arrest,
• Integration device - To allow integration and selection of our genetic constructs,

Here, AB is our antibiotic resistance cassette, ytvA is the gene controlling the light-sensing pathway, SB is the biomaterial, epsE the clutch and the 5' and 3' sections are integration sites. Light-inducible promoters are labelled with an 'L'.

Basically our dry lab team concentrated on characterising the chassis. In the dry lab section you'll find pages on the genetic circuit, growth curve and motility analysis from our project; this section will give a brief brief overview of each area (no pictures?). It will also include (in the motility analysis part) a movie (y/n?) of the motility response of B. subtilis with an inducible epsE gene BioBricked in.

Growth Curve

The growth curve of B. subtilis was modelled by superposition of three more basic ODE models, which were constructed and simulated in MATLAB. The lag, exponential and stationary phases are modelled and combined to produce the curve on the right (Image here?).

Genetic Circuit

We feel accurate modelling of the genetic circuit contributes greatly to the characterisation of synthetic systems. As part of the project, the behaviours of constitutive and inducible promoters were modelled for comparison with our experimental data.

Motility Analysis

A major component of the system is the motility and shift between a motile and arrested state with the expression of EpsE.

Think what we can refer to implementation as 1)the construction of the parts ,2) the work with B.subtilis integration3)growth curve if we want to. Idea is to have three sub boxes with a large picture and brief text underneath. Last week Kirsten had the idea that we could make a big gel with the digests from all our parts, I will look into this week to get an image. \We could use this then put some stuff about no. of parts etc. Think

Results from the B.subtilis motility and maybe the part characterisation

• Helped Bristol by sending them a mini-iGEM project: Chemotactic dot-to-dot with information on quorum sensing and directed movement
• Helped Bristol by sending them a part (BBa_J37015) from our 2007 stock which was an empty vector in the Registry
• Developed integration bricks, to allow devices to be constructed that can then be excised and planted into B. subtilis
• Layed the groundwork for future teams to work with B. subtilis by BioBricking promoters, RBSs, terminators and so on and characterising them
• Showed that expansion into other organisms is a definite possibility!

Of course, that's a very simplified description of our project. We expanded upon our project by looking into possible areas for real-world application; for a case-study of such an implementation check out how our project fits in with >>> Biocouture >>>