Waterloo/23 June 2008

From 2008.igem.org

08/06/23 Monday

Morning
[x] Mini prep cultures from Sunday (2007 and 2008 registry) 10:00 - 12:30
- 2007 done, but need to redo 2008 miniprep on Wednesday
[x] need to re-innoculate 2008 (samples were mixed up)
- Keep it at 4C tomrrow for wed to mini prep with 2007 registry parts.

[x] need more solution 1
[x] update 1-2-3 mini prep protocol

[x] Diagnostic digest of mini preps (12:45 - 1:30) and gel (1.5 hr)

- Look at DNA concentrations for previous ligations to trouble shoot low transformation efficiency of the ones that we have enough vol (11 out 14) all have concentrations > 30ng/uL

[x] elute the rest of the 2007 registry parts from the registry plate.
- One transformant didn't work, will be repeated