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Alberta NINT/31 July 2008
From 2008.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
lab work (JD): | lab work (JD): | ||
Counted colonies on both mine and Shannon's plates from yesterday's ligations | Counted colonies on both mine and Shannon's plates from yesterday's ligations | ||
| - | -unfortunately equal numbers on negative plates and ligation plates so ligation did not work | + | -unfortunately equal numbers on negative plates and ligation plates so ligation |
| - | -my plate had 100s on the negative control so I assumed the vector was not cut properly | + | did not work |
| + | -my plate had 100s on the negative control so I assumed the vector was not cut | ||
| + | properly | ||
Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel | Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel | ||
[[Alberta_NINT/1_August_2008 | Next entry >]] | [[Alberta_NINT/1_August_2008 | Next entry >]] | ||
Revision as of 18:18, 11 August 2008
lab work (JD):
Counted colonies on both mine and Shannon's plates from yesterday's ligations
-unfortunately equal numbers on negative plates and ligation plates so ligation
did not work
-my plate had 100s on the negative control so I assumed the vector was not cut
properly
Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel
