"
Assembly Related Protocols
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | <span style="font-family: Century gothic; font-size: 10pt"> | |
| + | |||
| + | ==Newest EcoRI/BamHI Transfer method (7/31/08)== | ||
Digestion: | Digestion: | ||
Revision as of 03:04, 2 August 2008
Newest EcoRI/BamHI Transfer method (7/31/08)
Digestion:
- 0.5ul plasmid in entry vector
- 0.5ul EcoRI
- 0.5ul BamHI
- 1ul NEB2 + ATP buffer
- 7.5ul of water
- incubate the digestion for 1hr. in the thermocycler, and 20 min for heat kill.
No purification step.
Ligation:
- 1ul of dilute assembly vector (or 0.5ul if undiluted)
- 0.5ul ligase
Transform. (same as usual)
