Assembly Related Protocols

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Newest EcoRI/BamHI Transfer method (7/31/08)

Digestion: 0.5ul plasmid in entry vector 0.5ul EcoRI 0.5ul BamHI 1ul NEB2 + ATP buffer 7.5ul of water incubate the digestion for 1hr. in the thermocycler, and 20 min for heat kill.

No purification step.

Ligation: 1ul of dilute assembly vector (or 0.5ul if undiluted) 0.5ul ligase

Transform. (same as usual)

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