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Beijing Normal/21 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: Results: The transformation of pSB1A3 is unsuccessful, however the situation of pSB1AT3 is no better: just 20~30 colonies. The steps as follows: 1.cut douwn the sports...) |
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| Line 1: | Line 1: | ||
Results: | Results: | ||
| - | The transformation of pSB1A3 is unsuccessful, however the situation of pSB1AT3 is no better: just 20~30 colonies. | + | The transformation of pSB1A3 is unsuccessful, |
| + | however the situation of pSB1AT3 is no better: just 20~30 colonies. | ||
The steps as follows: | The steps as follows: | ||
1.cut douwn the sports stricktly following the instructions provided and we do spin it. | 1.cut douwn the sports stricktly following the instructions provided and we do spin it. | ||
| Line 10: | Line 11: | ||
7 37 degree incubate for more than 60mins | 7 37 degree incubate for more than 60mins | ||
8 plate the incubated cell culture on the plate containing amp | 8 plate the incubated cell culture on the plate containing amp | ||
| - | 9 incubate the plate at 37 degree for 12~14h | + | 9 incubate the plate at 37 degree for 12~14h |
Questions: | Questions: | ||
| Line 17: | Line 18: | ||
2 Is 5ul TE enough for u to elute the plamids? Or the solution is all absorbed by the | 2 Is 5ul TE enough for u to elute the plamids? Or the solution is all absorbed by the | ||
the soaked paper? | the soaked paper? | ||
| - | 3 What does the instruction mean by 10% bleach? We have several kinds of bleach in the lab and are confused about which is proper. | + | 3 What does the instruction mean by 10% bleach? |
| + | We have several kinds of bleach in the lab and are confused about which is proper. | ||
Latest revision as of 04:52, 23 July 2008
Results:
The transformation of pSB1A3 is unsuccessful,
however the situation of pSB1AT3 is no better: just 20~30 colonies.
The steps as follows:
1.cut douwn the sports stricktly following the instructions provided and we do spin it.
2. add all the TE containing the plasmids to 50ul competence cell (Top10, tested before expet)
3 stock in the 4 degree frigde for 30mins
4 42 degree heat shock
5 4 degree stock for 5~6 mins
6 add 300ul SOB.
7 37 degree incubate for more than 60mins
8 plate the incubated cell culture on the plate containing amp
9 incubate the plate at 37 degree for 12~14h
Questions:
However, we wanna to ask other teams members: 1 When u eject the sport into the tube, will the colour turn pink? 2 Is 5ul TE enough for u to elute the plamids? Or the solution is all absorbed by the the soaked paper? 3 What does the instruction mean by 10% bleach? We have several kinds of bleach in the lab and are confused about which is proper.
