Beijing Normal/27 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: We and Tsinghua team share some experience on how to do the transformation of ligation product. We generalize some key words: 1. Make sure the concentration of the palsmids from the biobri...) |
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We and Tsinghua team share some experience on how to do the transformation of ligation product. | We and Tsinghua team share some experience on how to do the transformation of ligation product. | ||
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We generalize some key words: | We generalize some key words: | ||
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1. Make sure the concentration of the palsmids from the biobrick is high enough. | 1. Make sure the concentration of the palsmids from the biobrick is high enough. | ||
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2. Keep a loe temprature when enzyme digestion is performed | 2. Keep a loe temprature when enzyme digestion is performed | ||
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3. The transformation product should be incubated for more than 1h. | 3. The transformation product should be incubated for more than 1h. |
Latest revision as of 02:43, 29 August 2008
We and Tsinghua team share some experience on how to do the transformation of ligation product.
We generalize some key words:
1. Make sure the concentration of the palsmids from the biobrick is high enough.
2. Keep a loe temprature when enzyme digestion is performed
3. The transformation product should be incubated for more than 1h.