Beijing Normal/7 October 2008

From 2008.igem.org

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(New page: PCR amplification of T7 termitor was performed. The yeild is satisfying.)
 
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PCR amplification of T7 termitor was performed. The yeild is satisfying.
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PCR restrict digestion of T7 termitor and its vector was performed. We use double digestion system, BamH1 and XbaI in NEB Buffer 3. Xba1 was added more than BamH1. ligation was performed later. We cannot do the transformation today because there is no Top10 left in the fridge.

Latest revision as of 06:17, 8 October 2008

PCR restrict digestion of T7 termitor and its vector was performed. We use double digestion system, BamH1 and XbaI in NEB Buffer 3. Xba1 was added more than BamH1. ligation was performed later. We cannot do the transformation today because there is no Top10 left in the fridge.