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Colony stab
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Riachalder (Talk | contribs) (New page: * Pipette 10mL LB into red-capped culture tubes or conical shake flasks. * Using an autoclaved toothpick, gently touch the point to a single colony. * Drop the toothpick point down into ...) |
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| + | ==Making Overnight Cultures from Agar Plate Colonies== | ||
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* Pipette 10mL LB into red-capped culture tubes or conical shake flasks. | * Pipette 10mL LB into red-capped culture tubes or conical shake flasks. | ||
* Using an autoclaved toothpick, gently touch the point to a single colony. | * Using an autoclaved toothpick, gently touch the point to a single colony. | ||
* Drop the toothpick point down into the LB. | * Drop the toothpick point down into the LB. | ||
* Incubate overnight at 37°C whilst shaking. | * Incubate overnight at 37°C whilst shaking. | ||
| + | |||
| + | Back to [[Team:Newcastle University/Notebook]] | ||
Revision as of 11:43, 18 September 2008
Making Overnight Cultures from Agar Plate Colonies
- Pipette 10mL LB into red-capped culture tubes or conical shake flasks.
- Using an autoclaved toothpick, gently touch the point to a single colony.
- Drop the toothpick point down into the LB.
- Incubate overnight at 37°C whilst shaking.
Back to Team:Newcastle University/Notebook
