Colony stab

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(New page: * Pipette 10mL LB into red-capped culture tubes or conical shake flasks. * Using an autoclaved toothpick, gently touch the point to a single colony. * Drop the toothpick point down into ...)
 
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==Making Overnight Cultures from Agar Colonies==
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* Pipette 10mL LB into red-capped culture tubes or conical shake flasks.  
* Pipette 10mL LB into red-capped culture tubes or conical shake flasks.  
* Using an autoclaved toothpick, gently touch the point to a single colony.  
* Using an autoclaved toothpick, gently touch the point to a single colony.  
* Drop the toothpick point down into the LB.  
* Drop the toothpick point down into the LB.  
* Incubate overnight at 37°C whilst shaking.
* Incubate overnight at 37°C whilst shaking.
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Back to [[Team:Newcastle University/Notebook]]

Latest revision as of 11:43, 18 September 2008

Making Overnight Cultures from Agar Colonies

  • Pipette 10mL LB into red-capped culture tubes or conical shake flasks.
  • Using an autoclaved toothpick, gently touch the point to a single colony.
  • Drop the toothpick point down into the LB.
  • Incubate overnight at 37°C whilst shaking.

Back to Team:Newcastle University/Notebook