IGEM:Cambridge/2008/Notebook/Voltage/Flame Photometer Calibration
From 2008.igem.org
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- | + | =Potassium Assay= | |
- | =Method= | + | To get data about the internal potassim concentration of our cells under various growth conditions, they were lysed and then the solution fed through a flame photometer. |
+ | ==Method== | ||
*Cells are grown overnight in NOK containing various K+ concentrations | *Cells are grown overnight in NOK containing various K+ concentrations | ||
*OD600 data is taken for each sample | *OD600 data is taken for each sample | ||
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*Samples are then fed into flame photometer as [[Team:Cambridge/Protocols#Flame_Photometry| protocol]] | *Samples are then fed into flame photometer as [[Team:Cambridge/Protocols#Flame_Photometry| protocol]] | ||
- | =Results= | + | ==Results== |
Calibration for low concentrations: | Calibration for low concentrations: |
Revision as of 20:44, 29 October 2008
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Potassium AssayTo get data about the internal potassim concentration of our cells under various growth conditions, they were lysed and then the solution fed through a flame photometer. Method
ResultsCalibration for low concentrations: Calibration for high concentrations: presynaptic plasma membrane. The neurotransmitter diffuses through the synaptic cleft and binds to chemical receptor molecules on the membrane of the postsynaptic cell. These receptors cause ion channels to open so that ions rush out, changing the transmembrane potential. Attempting to mimic this in a prokaryotic system is particularly attractive as, in a more general sense, it provides an interface between chemical or biological and electrical systems.
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