Latest revision as of 20:49, 29 October 2008

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Flame Photometer Calibration

To calibrate the flame photometer various solutions of potassium were fed through it.

Calibration for low concentrations:

Calibration for high concentrations:

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-=Potassium Assay=
+=Flame Photometer Calibration=
-To get data about the internal potassim concentration of our cells under various growth conditions, they were lysed and then the solution fed through a flame photometer.
+To calibrate the flame photometer various solutions of potassium were fed through it.
 ==Method==
-*Cells are grown overnight in NOK containing various K+ concentrations
+*Solutions of KCl aremade up from stock 2M, by serial dilution
-*OD600 data is taken for each sample
-*2ml of cells are centrifuged at 13000g to pellet
-*Pellet resuspended in 2ml of NOK - no K+
-*Cells spun down again and resuspended in 2ml NOK
-*Tubes are frozen in liquid nitrogen and thawed in a waterbath. Repeat 3 times.
 *Samples are then fed into flame photometer as [[Team:Cambridge/Protocols#Flame_Photometry| protocol]]
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-presynaptic plasma membrane. The neurotransmitter diffuses through the synaptic cleft and binds to chemical receptor molecules on the membrane of the postsynaptic cell. These receptors cause ion channels to open so that ions rush out, changing the transmembrane potential. Attempting to mimic this in a prokaryotic system is particularly attractive as, in a more general sense, it provides an interface between chemical or biological and electrical systems.'''