IGEM:Cambridge/2008/Notebook/Voltage/Future Work
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+ | =Future Work= | ||
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+ | ==Better K+ uptake== | ||
+ | If a stronger K+ uptake could be made then in principal cells could be grown in the same medium as testing was done. This would potentially allow cells to be grown as a surface over an agar plate and still act to produce action potentials, thereby acting as neurones. | ||
+ | *Up regulate KDP constitutively | ||
+ | *Find K+ tolerant strains | ||
+ | *Check maximum trans-membrane potential | ||
+ | |||
+ | ==Move to [[Team:Cambridge/Bacillus|''Bacillus'']]== | ||
+ | Need to make GluR0 ''Bacillus'' compatible | ||
*Codon bias already favourable for ''Bacillus'' | *Codon bias already favourable for ''Bacillus'' | ||
+ | *Need to put different promoter and RBS | ||
+ | *Integrate into genome? | ||
+ | |||
+ | ==Voltage Gated Potassium Channel== | ||
+ | To get self propagating signal we need to find and construct a voltage gated potassium channel. | ||
+ | *Relatively rare in Prokaryotes | ||
+ | *Some available although not well characterised, such as Kch | ||
+ | *Overexpress and see if it improves output. | ||
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Latest revision as of 22:32, 29 October 2008
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Future WorkBetter K+ uptakeIf a stronger K+ uptake could be made then in principal cells could be grown in the same medium as testing was done. This would potentially allow cells to be grown as a surface over an agar plate and still act to produce action potentials, thereby acting as neurones.
Move to BacillusNeed to make GluR0 Bacillus compatible
Voltage Gated Potassium ChannelTo get self propagating signal we need to find and construct a voltage gated potassium channel.
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