IGEM:Cambridge/2008/Notebook/Voltage/Future Work

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=Future Work=
=Future Work=
==Better K+ uptake==
==Better K+ uptake==
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If a stronger K+ uptake could be made then in pricipal cells could be grown in the same medium as testing was done
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If a stronger K+ uptake could be made then in principal cells could be grown in the same medium as testing was done. This would potentially allow cells to be grown as a surface over an agar plate and still act to produce action potentials, thereby acting as neurones.
*Up regulate KDP constitutively
*Up regulate KDP constitutively
*Find K+ tolerant strains
*Find K+ tolerant strains
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==Voltage Gated Potassium Channel==
==Voltage Gated Potassium Channel==
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To get self propogating signal we need to find and construct a voltage gated potassium channel.
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To get self propagating signal we need to find and construct a voltage gated potassium channel.
*Relatively rare in Prokaryotes
*Relatively rare in Prokaryotes
*Some available although not well characterised, such as Kch
*Some available although not well characterised, such as Kch
*Overexpress and see if it improves output.
*Overexpress and see if it improves output.
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Latest revision as of 22:32, 29 October 2008

Home   Voltage   Signalling   Bacillus   Modelling   Protocols   People  
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Future Work

Better K+ uptake

If a stronger K+ uptake could be made then in principal cells could be grown in the same medium as testing was done. This would potentially allow cells to be grown as a surface over an agar plate and still act to produce action potentials, thereby acting as neurones.

  • Up regulate KDP constitutively
  • Find K+ tolerant strains
  • Check maximum trans-membrane potential

Move to Bacillus

Need to make GluR0 Bacillus compatible

  • Codon bias already favourable for Bacillus
  • Need to put different promoter and RBS
  • Integrate into genome?

Voltage Gated Potassium Channel

To get self propagating signal we need to find and construct a voltage gated potassium channel.

  • Relatively rare in Prokaryotes
  • Some available although not well characterised, such as Kch
  • Overexpress and see if it improves output.

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