IGEM:Cambridge/2008/Notebook/Voltage/Future Work

From 2008.igem.org

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(New page: =Move to ''Bacillus''= Need to make GluR0 bacillus compatible *Codon bias already favourable for ''Bacillus'')
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=Move to [[Team:Cambridge/Bacillus|''Bacillus'']]=
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=Future Work=
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Need to make GluR0 bacillus compatible
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==Better K+ uptake==
 +
If a stronger K+ uptake could be made then in pricipal cells could be grown in the same medium as testing was done
 +
*Up regulate KDP constitutively
 +
*Find K+ tolerant strains
 +
*Check maximum trans-membrane potential
 +
 
 +
==Move to [[Team:Cambridge/Bacillus|''Bacillus'']]==
 +
Need to make GluR0 ''Bacillus'' compatible
*Codon bias already favourable for ''Bacillus''
*Codon bias already favourable for ''Bacillus''
 +
*Need to put different promoter and RBS
 +
*Integrate into genome?
 +
 +
==Voltage Gated Potassium Channel==
 +
To get self propogating signal we need to find and construct a voltage gated potassium channel.
 +
*Relatively rare in Prokaryotes
 +
*Some available although not well characterised, such as Kch
 +
*Overexpress and see if it improves output.

Revision as of 20:28, 29 October 2008

Contents

Future Work

Better K+ uptake

If a stronger K+ uptake could be made then in pricipal cells could be grown in the same medium as testing was done

  • Up regulate KDP constitutively
  • Find K+ tolerant strains
  • Check maximum trans-membrane potential

Move to Bacillus

Need to make GluR0 Bacillus compatible

  • Codon bias already favourable for Bacillus
  • Need to put different promoter and RBS
  • Integrate into genome?

Voltage Gated Potassium Channel

To get self propogating signal we need to find and construct a voltage gated potassium channel.

  • Relatively rare in Prokaryotes
  • Some available although not well characterised, such as Kch
  • Overexpress and see if it improves output.
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