IGEM:Cambridge/2008/Notebook/Voltage/Future Work

From 2008.igem.org

(Difference between revisions)
(Move to Bacillus)
Line 1: Line 1:
 +
{{Cambridge/Notitle}}
 +
<div class=bodytable>
 +
{{Cambridge08}}
 +
{{Cambridge08voltage}}
 +
<html>
 +
<table style="background:#444444; padding:15px;">
 +
<table align=left width= border="0" style="background:#444444; padding:5px;">
 +
<tr>
 +
<td style="width:100%; height: 400; padding-left: 15px;">
 +
    <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>
 +
    <div class="contentf">
 +
    <div style="height: 400; background: white; line-height:170% padding: 5px;">
 +
<div style="color: white; font: 2px;">x</div>
 +
</html>
 +
=Future Work=
=Future Work=

Revision as of 20:29, 29 October 2008

x

Contents

Future Work

Better K+ uptake

If a stronger K+ uptake could be made then in pricipal cells could be grown in the same medium as testing was done

  • Up regulate KDP constitutively
  • Find K+ tolerant strains
  • Check maximum trans-membrane potential

Move to Bacillus

Need to make GluR0 Bacillus compatible

  • Codon bias already favourable for Bacillus
  • Need to put different promoter and RBS
  • Integrate into genome?

Voltage Gated Potassium Channel

To get self propogating signal we need to find and construct a voltage gated potassium channel.

  • Relatively rare in Prokaryotes
  • Some available although not well characterised, such as Kch
  • Overexpress and see if it improves output.