IGEM:Cambridge/2008/Notebook/Voltage/K+ Growth

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Latest revision as of 17:49, 29 October 2008

Home   Voltage   Signalling   Bacillus   Modelling   Protocols   People  
Overview   Constructs   Mutants   Potassium Assay   Voltage Output   Future Work  

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Method

  • For each of the 9 mutants, 3 [K+] of growth medium were examined: 1mM, 50mM & 200mM

Composition of Each Cuvette: 

                     * 100ul cells
                     * 100ul KCl (10x stocks)
                     * 800ul LB 
                       ----------------------
                         1ml Final Volume
  • The spectrophotometer was zeroed using LB.
  • OD readings were taken every 30 minutes for 3 hours.
  • In between readings, cultures were stored at room temperature & covered with parafilm.

Results

Link to raw OD600 Data in Excel

RelGrowth1mmK.JPG

RelGrowth50mmK.JPG

RelGrowth200mmK.JPG


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