IGEM:Cambridge/2008/Notebook/Voltage/OD600 Calibration

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(New page: =Method= *An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.) *Dilutions were plated out at 10<sup>-5</s...)
 
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=Calibration Method=
*An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.)
*An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.)
*Dilutions were plated out at 10<sup>-5</sup> and 10<sup>-6</sup> fold dilutions, and colonies were counted using a colony counter to determine the total number of cells in the original dilutions.
*Dilutions were plated out at 10<sup>-5</sup> and 10<sup>-6</sup> fold dilutions, and colonies were counted using a colony counter to determine the total number of cells in the original dilutions.
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Latest revision as of 20:04, 29 October 2008

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OD600

Calibration Method

  • An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.)
  • Dilutions were plated out at 10-5 and 10-6 fold dilutions, and colonies were counted using a colony counter to determine the total number of cells in the original dilutions.

Results

Relative Cell Density OD600 Reading Colonies on x100,000 dilution
1 1.6483 745
0.8 1.4913
0.6 1.1058
0.4 1.0167 308
0.2 0.5885
0.1 0.2982
0.08 0.2567
0.06 0.1910
0.04 0.1208
0.02 0.070
0.01 0.0346

0D600 curve.JPG


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