IGEM:Cambridge/2008/Notebook/Voltage/Output

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=Setup=
=Setup=
Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.
Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.
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=Results=
=Results=
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*With the setup above a voltage change could be observed on the oscilloscope.
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*The following results were recorded by adding different amounts of 10uL glutamate to the samples.
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  *10uL Aspartate produced the bottom curve, showing very little recover
 +
  *5uL Glutamate gave the next curve
 +
  *10uL Glutamate gave the third curve
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  *20uL Glutamate gave the best recovery, reaching the highest level
[[Image:Voltage_Response.jpg |300px | Output at different GluR0 concentrations]]
[[Image:Voltage_Response.jpg |300px | Output at different GluR0 concentrations]]

Revision as of 17:06, 29 October 2008

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Setup

Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.

  • Cells containing biobrick K090002 are grown overnight in NOK with 5%(by vol.) 2M KCl added - final 100mM K+.
  • Samples are then spun down in individual 1.5ml eppendorfs.

Experiment

  • Resuspend cells in 200μL clean NOK and place in sample wells
  • Lower probe electrode into cell solution and wait for stable reading - 1min.
  • Add sample solution

Apparatus setup

Results

  • With the setup above a voltage change could be observed on the oscilloscope.
  • The following results were recorded by adding different amounts of 10uL glutamate to the samples. 
 *10uL Aspartate produced the bottom curve, showing very little recover
 *5uL Glutamate gave the next curve
 *10uL Glutamate gave the third curve
 *20uL Glutamate gave the best recovery, reaching the highest level

Output at different GluR0 concentrations

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