Imperial College/10 September 2008

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10 September 2008

Wet Lab

Transformation

Growth Curve

We performed an OD600 against time growth curve for B. subtilis. The results are shown below:


OD600 v Time.PNG

Please click on the link below for the Excel data file:
Media:OD 600 v Time.xls

Cloning

  • All ligations from yesterday failed, no indication of why. 2 test ligations set up and used to transfom Xl1-blue. *Overnight ligations set up for transformation tomorrow
  • Constructs 3, 6, 10 and 12 midiprepped

Dry Lab

Motility

Took 20 videos, binned x4 to a frame rate of 27.9fps, more than twice the rate at which previous videos were taken. However, video quality is not as good as before, but cells can still be easily distinguished.

Growth Curve

Obtained sigmoidal curve for bacteria growth using MATLAB.