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Imperial College/11 August 2008
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Biofabricator Subtilis
Project Specifications
Why B. subtilis?
B. subtilis: Benefits vs Challenges
Project Summary
Example Application:
Biocouture
Dry Lab
Dry Lab Hub
Growth Curves
Genetic Circuits
Motility Analysis
Appendices - Code etc.
Wet Lab
Wet Lab Hub
Cloning Strategy
Experiments & Protocols
BioBricks Submitted
Experimental Results
Notebook
Our Team
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11 August 2008
Wetlab Team
B.subtilis
Today made up LB broth, SOC broth and PEG 6000.
We inoculated the following overnight cultures:
5mlx3 from pDR110
E.coli
DH5 aplha,
5mlx3 from pDR111
E.coli
DH5 aplha,
10ml from
B.subtilis
Cloning
Finished the cloning scheme
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