Imperial College/11 September 2008
From 2008.igem.org
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*On the issue of which cells to track, we have decided to ignore dead cells, but we must take note of the % of cells with swimming motility. This excludes cells which drift. We have defined cells with swimming motility as '''cells which travel in the direction of their longitudinal axis'''. | *On the issue of which cells to track, we have decided to ignore dead cells, but we must take note of the % of cells with swimming motility. This excludes cells which drift. We have defined cells with swimming motility as '''cells which travel in the direction of their longitudinal axis'''. | ||
*More cells will be tracked tomorrow and over the weekend. | *More cells will be tracked tomorrow and over the weekend. | ||
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+ | ==Wet Lab== | ||
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+ | *Constructs 3, 6, 10 and 12 digested with ''Eco''RI and ''Spe''I ready for gel purification | ||
+ | *Overnight ligations from yesterday transformed into Xl1-blue ''E. coli''. | ||
+ | *Yesterday's bench ligations used to transform Xl1-blues failed to grow | ||
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{{Imperial/EndPage}} | {{Imperial/EndPage}} |
Revision as of 00:23, 12 September 2008
Dry Lab
Wet Lab
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