Imperial College/13 August 2008

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*Make LB agar plates (at least 6) containing streptinomycin
*Make LB agar plates (at least 6) containing streptinomycin
*Prepare a ''B.subtilis'' culture for the microscope - Overnight culture then resuspend in the morning
*Prepare a ''B.subtilis'' culture for the microscope - Overnight culture then resuspend in the morning
 +
*Perform electroporation and pate out transformants

Revision as of 21:22, 12 August 2008

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13 August 2008

B.subtilis

  • Run the mini prep of the two integration vectors pDR110/pDR111 on a agarose gel to check purity and quantity
  • Make LB agar plates (at least 6) containing streptinomycin
  • Prepare a B.subtilis culture for the microscope - Overnight culture then resuspend in the morning
  • Perform electroporation and pate out transformants
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