Revision as of 16:09, 13 August 2008

13 August 2008

The two minipreps of the pDR110 and pDR111 were run on a gel electrophoresis. 8ul of each DNA was combined with 4ul of sample buffer and run on a gel.(image needs to be uploaded),
100ug/ml Spectinmycin LB agar plates were prepared,
Ampicillin, kanaomycin and spectinomycin aliquots were prepared today,

@@ Line 9: / Line 9: @@
 = 13 August 2008 =
-==''B.subtilis''==
+==Wet Lab==
-*Run the mini prep of the two integration vectors pDR110/pDR111 on a agarose gel to check purity and quantity
+*The two minipreps of the pDR110 and pDR111 were run on a gel electrophoresis. 8ul of each DNA was combined with 4ul of sample buffer and run on a gel.(image needs to be uploaded),
-*Make LB agar plates (at least 6) containing streptinomycin
+*100ug/ml Spectinmycin LB agar plates were prepared,
-*Prepare a ''B.subtilis'' culture for the microscope - Overnight culture then resuspend in the morning
+*Ampicillin, kanaomycin and spectinomycin aliquots were prepared today,
-*Perform electroporation and pate out transformants