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Home
Biofabricator Subtilis
Project Specifications
Why B. subtilis?
B. subtilis: Benefits vs Challenges
Project Summary
Example Application:
Biocouture
Dry Lab
Dry Lab Hub
Growth Curves
Genetic Circuits
Motility Analysis
Appendices - Code etc.
Wet Lab
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Cloning Strategy
Experiments & Protocols
BioBricks Submitted
Experimental Results
Notebook
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13 August 2008
B.subtilis
Run the mini prep of the two integration vectors pDR110/pDR111 on a agarose gel to check purity and quantity
Make LB agar plates (at least 6) containing streptinomycin
Prepare a
B.subtilis
culture for the microscope - Overnight culture then resuspend in the morning
Perform electroporation and pate out transformants
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