8 Minipreps were carried out for samples G7-1, G7-2, G8-1, G9-1, G9-3, G10-3, G11-2 and G11-3
Miniprep Digestion
All the mini-preps were digested with EcoRI and PstI
U = undigested, D = digested
All digested bands appear to be approximately the right size, no trace of contamiantion beyond occaisonal bits of denatured DNA
Minipreps of PgsiB-gsiB, PgsiB-spoVG and Pveg-spoVG were digested with EcoRI and PstI and run on a gel along with uncut DNA from the minipreps of GeneArt constructs 4 and 5
U = undigested, D = digested
The sizes of inserts for PgsiB-gsiB and PgsiB-spoVG appear correct and the mini-preps seem fairly pure. No insert was cut from the Pveg-spoVG minipreps and they will need to be re-ligated for transformation
Dry Lab
Modelling Motility
Managed to run trajectory modelling on 90 cells yesterday.
Manual segmentation of runs done today to obtain a better curve fit. Hence certain cells will have two or more runs, and two or more parameter sets respectively.
Example is shown with 100908 Video 15 Cell 6. Notice that the model for a single run does not fit the data properly. Hence we introduce a change in orientation (parameters) at frame 75. This results in two separate runs, each with its respective set of parameters A, B and alpha.