Imperial College/7 August 2008

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7 August 2008

  • Chris and James grew up competent E. coli XL1-Blue cells for use in cloning Biobricks and assembling constructs.
  • B.subtilis strain 168 and E.coil DH5α containing plasmids pDR110 and pDR111 picked up from Dr. Gründling

Debrief

  • Laboratory induction tomorrow at 11am.
  • Wiki coming along but needs to be cut down in size. Tom will do this tomorrow and on the weekend.
  • Parts were all sent off; now that constructs ordered are finalised, we can use these and the test constructs to work out our critical pathway for cloning. Tom and Chris will make a cloning strategy tomorrow after the lab induction.
  • Dry lab team continues through tutorials...
  • Microscope training organised. Associated parties will need to fill out forms and training should take about an hour (2 on 1).