http://2008.igem.org/wiki/index.php?title=Imperial_College/9_September_2008&feed=atom&action=historyImperial College/9 September 2008 - Revision history2024-03-29T04:41:13ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=Imperial_College/9_September_2008&diff=81900&oldid=prevQiqin at 20:45, 28 October 20082008-10-28T20:45:38Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:45, 28 October 2008</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 41:</td>
<td colspan="2" class="diff-lineno">Line 41:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Conditions and timings were kept the same as the standard Taq protocol. Pfu PCR reactions using the optimal condiitons can now be set up to produce clones for use in our constructs. From the LacI and pSB1AK3 digests, it is now possible to decide how much of each is required in our ligation reaction.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Conditions and timings were kept the same as the standard Taq protocol. Pfu PCR reactions using the optimal condiitons can now be set up to produce clones for use in our constructs. From the LacI and pSB1AK3 digests, it is now possible to decide how much of each is required in our ligation reaction.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{Imperial/EndPage}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{Imperial/EndPage<ins class="diffchange diffchange-inline">|Notebook|Notebook</ins>}}</div></td></tr>
</table>Qiqinhttp://2008.igem.org/wiki/index.php?title=Imperial_College/9_September_2008&diff=48039&oldid=prevChris at 10:43, 25 September 20082008-09-25T10:43:16Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 10:43, 25 September 2008</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| rowspan="2" bgcolor=#ffffff width="100%" |</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| rowspan="2" bgcolor=#ffffff width="100%" |</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=<del class="diffchange diffchange-inline">8 </del>September 2008=</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=<ins class="diffchange diffchange-inline">9 </ins>September 2008=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Chrishttp://2008.igem.org/wiki/index.php?title=Imperial_College/9_September_2008&diff=45072&oldid=prevQiqin at 22:16, 12 September 20082008-09-12T22:16:52Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:16, 12 September 2008</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{Imperial/StartPage}}__NOTOC__</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{| cellpadding="10" border="0"</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|- valign="top"</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|{{#calendar: title=Imperial_College |year=2008 | month=08}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|{{#calendar: title=Imperial_College |year=2008 | month=09}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|{{#calendar: title=Imperial_College |year=2008 | month=10}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">| rowspan="2" bgcolor=#ffffff width="100%" |</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=8 September 2008=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=8 September 2008=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 31:</td>
<td colspan="2" class="diff-lineno">Line 39:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lane 11 - pSB1AK3 vector (cut with ''Xba''I and ''Spe''I)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lane 11 - pSB1AK3 vector (cut with ''Xba''I and ''Spe''I)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Conditions and timings were kept the same as the standard Taq protocol. Pfu PCR reactions using the optimal condiitons can now be set up to produce clones for use in our constructs. From the LacI and pSB1AK3 digests, it is now possible to decide how much of each is required in our ligation reaction</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Conditions and timings were kept the same as the standard Taq protocol. Pfu PCR reactions using the optimal condiitons can now be set up to produce clones for use in our constructs. From the LacI and pSB1AK3 digests, it is now possible to decide how much of each is required in our ligation reaction<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{Imperial/EndPage}}</ins></div></td></tr>
</table>Qiqinhttp://2008.igem.org/wiki/index.php?title=Imperial_College/9_September_2008&diff=44851&oldid=prevChris: /* Wet Lab */2008-09-12T11:39:17Z<p><span class="autocomment">Wet Lab</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 11:39, 12 September 2008</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 9:</td>
<td colspan="2" class="diff-lineno">Line 9:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:9-08PCR.PNG|thumb|400px|center|A 1% Agarose gel showing the results of various PCR reactions. Each lane is loaded with 5ul of PCR reaction and 1ul of 6x sample buffer. M = Marker]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:9-08PCR.PNG|thumb|400px|center|A 1% Agarose gel showing the results of various PCR reactions. Each lane is loaded with 5ul of PCR reaction and 1ul of 6x sample buffer. M = Marker]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 1 - EpsE 5' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 1 - EpsE 5' IS primers, chromosome template, <ins class="diffchange diffchange-inline">52</ins><sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 2 - EpsE 5' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 2 - EpsE 5' IS primers, chromosome template, <ins class="diffchange diffchange-inline">50</ins><sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 3 - EpsE 5' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 3 - EpsE 5' IS primers, chromosome template, <ins class="diffchange diffchange-inline">48</ins><sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 4 - XylR primers, chromosome template, <sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 4 - XylR primers, chromosome template, <ins class="diffchange diffchange-inline">50</ins><sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 5 - XylR primers, chromosome template, <sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 5 - XylR primers, chromosome template, <ins class="diffchange diffchange-inline">48</ins><sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 6 - XylR primers, chromosome template, <sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 6 - XylR primers, chromosome template, <ins class="diffchange diffchange-inline">46</ins><sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 7 - EpsE 3' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 7 - EpsE 3' IS primers, chromosome template, <ins class="diffchange diffchange-inline">54</ins><sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 8 - EpsE 3' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 8 - EpsE 3' IS primers, chromosome template, <ins class="diffchange diffchange-inline">52</ins><sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 9 - EpsE 3' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Lane 9 - EpsE 3' IS primers, chromosome template, <ins class="diffchange diffchange-inline">50</ins><sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lane 10 - LacI insert (cut with ''Xba''I and ''Spe''I)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lane 10 - LacI insert (cut with ''Xba''I and ''Spe''I)</div></td></tr>
</table>Chrishttp://2008.igem.org/wiki/index.php?title=Imperial_College/9_September_2008&diff=44735&oldid=prevChris: New page: =8 September 2008= =='''Wet Lab'''== *Taq PCR trials of genomic sequences set up and results analysed on gel *Ligations of all available sequences into biobricks set up [[Image:9-08PCR...2008-09-12T00:14:43Z<p>New page: =8 September 2008= =='''Wet Lab'''== *Taq PCR trials of genomic sequences set up and results analysed on gel *Ligations of all available sequences into biobricks set up [[Image:9-08PCR...</p>
<p><b>New page</b></p><div>=8 September 2008=<br />
<br />
<br />
=='''Wet Lab'''==<br />
<br />
*Taq PCR trials of genomic sequences set up and results analysed on gel<br />
*Ligations of all available sequences into biobricks set up<br />
<br />
[[Image:9-08PCR.PNG|thumb|400px|center|A 1% Agarose gel showing the results of various PCR reactions. Each lane is loaded with 5ul of PCR reaction and 1ul of 6x sample buffer. M = Marker]]<br />
<br />
Lane 1 - EpsE 5' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles<br />
<br />
Lane 2 - EpsE 5' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles<br />
<br />
Lane 3 - EpsE 5' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles<br />
<br />
Lane 4 - XylR primers, chromosome template, <sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles<br />
<br />
Lane 5 - XylR primers, chromosome template, <sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles<br />
<br />
Lane 6 - XylR primers, chromosome template, <sup>o</sup>C for 10 cycles then 60<sup>o</sup>C for 20 cycles<br />
<br />
Lane 7 - EpsE 3' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles<br />
<br />
Lane 8 - EpsE 3' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles<br />
<br />
Lane 9 - EpsE 3' IS primers, chromosome template, <sup>o</sup>C for 10 cycles then 64<sup>o</sup>C for 20 cycles<br />
<br />
Lane 10 - LacI insert (cut with ''Xba''I and ''Spe''I)<br />
<br />
Lane 11 - pSB1AK3 vector (cut with ''Xba''I and ''Spe''I)<br />
<br />
Conditions and timings were kept the same as the standard Taq protocol. Pfu PCR reactions using the optimal condiitons can now be set up to produce clones for use in our constructs. From the LacI and pSB1AK3 digests, it is now possible to decide how much of each is required in our ligation reaction</div>Chris