Judging/Variance/U. Michigan

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Dear iGEM Judges,
Dear iGEM Judges,
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I'm writing on behalf of 2008 Peking University iGEM Team to ask for permission to send non-biobrick parts to the registry for the jamboree.  
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I am writing on behalf of the University of Michigan Synthetic Biology 
 +
team to request your permission to submit non-BioBrick parts to the
 +
registry.
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As we can not find effective vectors for yeast expression in the registry, our team has been using the pGREG series of plasmids for yeast protein expression. We have to use the promoter which is sensitive to lacI or Gal4, so those yeast plasmids in the registry can not serve this function. For the same reason our parts can not flank the four standard restriction sites since our vector contains these four or at least some of these four. Of course, we are trying to standardize the parts we have constructed but it seems the time is limited. We wonder if we can send those plasmids based on PGREG backbone.  
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For iGEM 2008, our team is trying to build a synthetic clock in E.coli 
 +
that is an alteration of one found in mammals. However, upon review 
 +
of clock literature, we realized that our clock has more potential to
 +
oscillate if we reduce the noise in the system.  Hence, we want to 
 +
incorporate our genetic operons into the E.coli chromosome.  Our tool 
 +
for doing this are landing pads.
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Moreover, since our project has something to do the precise spot mutation of gal4, those plasmids contain gal4 or gal4 delta gene can not be standardized. As gal4 gene also contain some of the four restriction sites.  
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The two landing pads we are using for the project will replace the
 +
arabinose and leucine operons.  The leucine landing pad is a construct 
 +
that Dong Eun Chang, a former member of the Ninfa lab created, and the 
 +
arabinose landing pad was one of the projects we presented at iGEM 
 +
last year.  These landing pads are BioBrick compatible, so standard 
 +
BioBrick assembly can be used to construct into these plasmids. We 
 +
are in the process of writing up and uploading the documentation for 
 +
these parts on our wiki.
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Another thing that should be mentioned is that our gene hAIDsc is a codon optimized gene kindly offered by Pro. Youri. I am not sure whether he permits us to send this part to open source.
 
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Thank you very much!
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Thank you for your time and consideration,
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Amrit Misra
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Zhou Zhou
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College of Life Sciences, Peking University
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Beijing, 100871
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P.R.China
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mikezzchina@gmail.com
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===Response===
===Response===

Latest revision as of 12:13, 9 October 2008

Request

Dear iGEM Judges,

I am writing on behalf of the University of Michigan Synthetic Biology team to request your permission to submit non-BioBrick parts to the registry.

For iGEM 2008, our team is trying to build a synthetic clock in E.coli that is an alteration of one found in mammals. However, upon review of clock literature, we realized that our clock has more potential to oscillate if we reduce the noise in the system. Hence, we want to incorporate our genetic operons into the E.coli chromosome. Our tool for doing this are landing pads.

The two landing pads we are using for the project will replace the arabinose and leucine operons. The leucine landing pad is a construct that Dong Eun Chang, a former member of the Ninfa lab created, and the arabinose landing pad was one of the projects we presented at iGEM last year. These landing pads are BioBrick compatible, so standard BioBrick assembly can be used to construct into these plasmids. We are in the process of writing up and uploading the documentation for these parts on our wiki.


Thank you for your time and consideration, Amrit Misra

Response

Amit,

Thanks for your email. Request approved. Please carefully document how others can use your landing pads and provide the parts to the Registry in a format that will support ready reuse by others. Please also make sure to enter descriptions and instruction for using the parts directly into the Parts pages too.

Best, Drew and the iGEM Judging team

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