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Judging/Variance/U. Michigan
From 2008.igem.org
Request
Response
On Oct 8, 2008, at 11:18 PM, Zhou Zhou wrote:
Dear iGEM Judges,
I'm writing on behalf of 2008 Peking University iGEM Team to ask for permission to send non-biobrick parts to the registry for the jamboree.
As we can not find effective vectors for yeast expression in the registry, our team has been using the pGREG series of plasmids for yeast protein expression. We have to use the promoter which is sensitive to lacI or Gal4, so those yeast plasmids in the registry can not serve this function. For the same reason our parts can not flank the four standard restriction sites since our vector contains these four or at least some of these four. Of course, we are trying to standardize the parts we have constructed but it seems the time is limited. We wonder if we can send those plasmids based on PGREG backbone.
Moreover, since our project has something to do the precise spot mutation of gal4, those plasmids contain gal4 or gal4 delta gene can not be standardized. As gal4 gene also contain some of the four restriction sites.
Another thing that should be mentioned is that our gene hAIDsc is a codon optimized gene kindly offered by Pro. Youri. I am not sure whether he permits us to send this part to open source.
Thank you very much!
Zhou Zhou College of Life Sciences, Peking University Beijing, 100871 P.R.China mikezzchina@gmail.com
