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Molecular Cloning
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== Template Resources == | == Template Resources == | ||
| - | PhbCAB: PBHR68 Plasmid | + | PhbCAB: PBHR68 Plasmid<br> |
| - | Lac I: PET28a | + | Lac I: PET28a<br> |
| - | CRE: | + | CRE:<br> |
| - | PpPhaP: Ralstonia eutropha H16 genome | + | PpPhaP: Ralstonia eutropha H16 genome<br> |
| - | PrPhaR: Ralstonia eutropha H16 genome | + | PrPhaR: Ralstonia eutropha H16 genome<br> |
| - | SRRz: | + | SRRz: <br> |
EGFP: | EGFP: | ||
| + | <br> | ||
== Primers == | == Primers == | ||
Revision as of 13:51, 29 October 2008
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Molecular Cloning
Contents |
Plasmids
1 pUCPhbCAB
2 pACYC(lacI+) DuetSRRz
3 pACYC(lacI+) DuetEGFP
4 pACYC(lacI-) DuetSRRz
5 pACYC(lacI-) DuetEGFP
6 pACYC(LacI-)DuetSRRzPpPhaPLacILVACret7terPrPhaRLoxPLacILoxP
7 pACYC(LacI-)DuetEGFPPpPhaPLacILVACret7terPrPhaRLoxPLacILoxP
Template Resources
PhbCAB: PBHR68 Plasmid
Lac I: PET28a
CRE:
PpPhaP: Ralstonia eutropha H16 genome
PrPhaR: Ralstonia eutropha H16 genome
SRRz:
EGFP:
Primers
PCR
Fusion PCR
Cut and Insert
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