Paris/July 13
From 2008.igem.org
(Difference between revisions)
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* Biobricks 2008 : use of the maximum recoverable volume minipreps | * Biobricks 2008 : use of the maximum recoverable volume minipreps | ||
* Biobricks 2007 : use of 3µl of minipreps | * Biobricks 2007 : use of 3µl of minipreps | ||
- | |||
{| border="1" | {| border="1" | ||
- | | | + | |colspan="2" |'''Biobricks 2007''' |
- | |align="center"| | + | |align="center"|Strain |
- | |align="center"| | + | |align="center"|Transformation |
- | + | ||
|- | |- | ||
- | |colspan="4" |Biobricks 2008 | + | |MP1 (= J23100) |
+ | |ok | ||
+ | |- | ||
+ | |MP2 (= J23107) | ||
+ | |ok | ||
+ | |- | ||
+ | |MP3 (= B0015) | ||
+ | |ok | ||
+ | |- | ||
+ | |MP5 (= JB0030) | ||
+ | |ok | ||
+ | |- | ||
+ | |MP6 (= E0422) | ||
+ | |ok | ||
+ | |} | ||
+ | |||
+ | |||
+ | {| border="1" | ||
+ | |colspan="4" |'''Biobricks 2008''' | ||
+ | |align="center"|details | ||
+ | |align="center"|Strain | ||
+ | |align="center"|Antibiotics | ||
+ | |align="center"|Plate 2007 | ||
|- | |- | ||
- | | | + | |2E_SP 1000 |
- | + | |B0034 | |
- | |B0034 | + | |A |
|3O P1 | |3O P1 | ||
|- | |- | ||
- | | | + | |10F_SP 1001 |
- | + | |R0079 | |
- | |R0079 | + | |A |
|19N P1 | |19N P1 | ||
|- | |- | ||
- | | | + | |4F_SP 1013 |
- | + | |C0079 | |
- | |C0079 | + | |K |
|7K P2 | |7K P2 | ||
|- | |- | ||
- | | | + | |6H_SP 1001 |
- | + | |C0179 | |
- | |C0179 | + | |A |
|6H P1 | |6H P1 | ||
|- | |- | ||
- | | | + | |6D_SP 1007 |
- | + | |S03879 | |
- | |S03879 | + | |A |
| / | | / | ||
|- | |- | ||
- | | | + | |6B_SP 1001 |
- | + | |S03154 | |
- | |S03154 | + | |A |
|24K P1 | |24K P1 | ||
|- | |- | ||
- | | | + | |10F_SP 1002 |
- | + | |J23101 | |
- | |J23101 | + | |A |
|21G P3 | |21G P3 | ||
|- | |- | ||
- | | | + | |11F_SP 1002 |
- | + | |J23109 | |
- | |J23109 | + | |A |
|23G P3 | |23G P3 | ||
|- | |- | ||
- | | | + | |4C_SP 1000 |
- | + | |R0040 | |
- | |R0040 | + | |A |
|7O P1 | |7O P1 | ||
|- | |- | ||
- | | | + | |2G_SP 1000 |
- | + | |C0040 | |
- | |C0040 | + | |A |
|5C P1 | |5C P1 | ||
|- | |- | ||
- | | | + | |1C_SP 1008 |
- | + | |PSB1A3 | |
- | |PSB1A3 | + | |A |
|23E P1 | |23E P1 | ||
|- | |- | ||
- | | | + | |7G_SP 1013 |
- | + | |E1010 | |
- | |E1010 | + | |K |
|15M P2 | |15M P2 | ||
|- | |- | ||
- | | | + | |7E_SP 1001 |
- | + | |E0040 | |
- | |E0040 | + | |A |
|5H P1 | |5H P1 | ||
|- | |- | ||
- | | | + | |2F_SP 1016 |
- | + | |E0030 | |
- | |E0030 | + | |K |
|7G P1 | |7G P1 | ||
|} | |} |
Revision as of 18:28, 15 July 2008
delete
Preparation of Competent Cells
- Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
- Culture at 37°C until DO=0,57 (around 3h)
- Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
- Spin during 8', 2000rpm, 4°C
- Resuspension of the cell's pellet in 20mL of cold CaCl2 (0,1M) during 30' in ice
- Spin during 8', 2000rpm, 4°C
- Resuspension of the cell's pellet in 1mL of cold CaCl2 (0,1M)
- 1h of delay unexpected (problem with DNA lyophilized)
- Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C durin 40")
- Incubation O/N at 37°C of the transformed bacteria
- Biobricks 2008 : use of the maximum recoverable volume minipreps
- Biobricks 2007 : use of 3µl of minipreps
Biobricks 2007 | Strain | Transformation | |
MP1 (= J23100) | ok | ||
MP2 (= J23107) | ok | ||
MP3 (= B0015) | ok | ||
MP5 (= JB0030) | ok | ||
MP6 (= E0422) | ok |
Biobricks 2008 | details | Strain | Antibiotics | Plate 2007 | |||
2E_SP 1000 | B0034 | A | 3O P1 | ||||
10F_SP 1001 | R0079 | A | 19N P1 | ||||
4F_SP 1013 | C0079 | K | 7K P2 | ||||
6H_SP 1001 | C0179 | A | 6H P1 | ||||
6D_SP 1007 | S03879 | A | / | ||||
6B_SP 1001 | S03154 | A | 24K P1 | ||||
10F_SP 1002 | J23101 | A | 21G P3 | ||||
11F_SP 1002 | J23109 | A | 23G P3 | ||||
4C_SP 1000 | R0040 | A | 7O P1 | ||||
2G_SP 1000 | C0040 | A | 5C P1 | ||||
1C_SP 1008 | PSB1A3 | A | 23E P1 | ||||
7G_SP 1013 | E1010 | K | 15M P2 | ||||
7E_SP 1001 | E0040 | A | 5H P1 | ||||
2F_SP 1016 | E0030 | K | 7G P1 |