Project
From 2008.igem.org
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<td width="408" bgcolor="#03438A"><p class="STYLE19"><strong>Competition</strong></p> | <td width="408" bgcolor="#03438A"><p class="STYLE19"><strong>Competition</strong></p> | ||
- | <p class="STYLE15"> In the culture that both ampicillin and chloromycetin are available, it requires the expression of the both | + | <p class="STYLE15"> In the culture that both ampicillin and chloromycetin are available, it requires the expression of the both resistant genes for both antibiotics for a strain’s survival. Without adding any signal molecular as the initially inducing factor into the culture, the two kinds of E.coli can not communicate with each other so they will keep on the competitive stage. In this stage each kind of cell must survive all by it self in some method as assimilating the nutrition in the culture. As a result the two different kinds of E.coli fight with each other for the space and nutrient ingredient. </p> |
- | <p class="STYLE15">By adding some AHL into the culture, the | + | <p class="STYLE15">By adding some AHL into the culture, the luxPr promoter will get activated by the AHL and LuxR complex. And then the expression product of the rhlI gene, BHL will diffuse into the cell 2 which can sense BHL-RhIR complex by binding to the PrhI promoter and turning on the expression of luxI kanR and lacI genes. The LacI protein will bind to the PBad/araC promoter and therefore stop the digestion of the signal molecular by the expression of the aiiA gene. At the same time the expression of the luxI gene will send out AHL. By using a very similar mechanism the cell 1 can sense the AHL molecular.</p></td> |
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<td bgcolor="#03438A"><p class="STYLE19"><strong>Mutualism</strong> <br> | <td bgcolor="#03438A"><p class="STYLE19"><strong>Mutualism</strong> <br> | ||
In this state the two kinds of cells communicate with each other by sensing the signal molecular sent by the counterpart.<br> | In this state the two kinds of cells communicate with each other by sensing the signal molecular sent by the counterpart.<br> | ||
- | It seems that with the help of each other both of them can live better in the harsh environment and the fact is the capacity of the LuxPr and PrhI are higher | + | It seems that with the help of each other, both of them can live better in the harsh environment and the fact is the capacity of the LuxPr and PrhI are higher than that of the Plac and Pbad/araC promoters. With higher expression of the ampicillin and chloromycetin resistant protein both of them can survive in the ultra-high antibiotic concentration. </p></td> |
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<p><span class="STYLE9" style="margin-bottom: 0">This idea was inspired by the theory of Prisoner’s Dilemma. | <p><span class="STYLE9" style="margin-bottom: 0">This idea was inspired by the theory of Prisoner’s Dilemma. | ||
- | As in prisoners’ dilemma, the bacteria in our design are faced with two solutions for coexistence, they could either choose to cooperate with | + | As in prisoners’ dilemma, the bacteria in our design are faced with two solutions for coexistence, they could either choose to cooperate with each other by providing inducers to express their partners’ antibiotics-resistance genes or they could take a foe strategy in which no cooperation is needed for both strains’ survival.</span></p> |
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Revision as of 09:57, 29 October 2008
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This idea was inspired by the theory of Prisoner’s Dilemma. As in prisoners’ dilemma, the bacteria in our design are faced with two solutions for coexistence, they could either choose to cooperate with each other by providing inducers to express their partners’ antibiotics-resistance genes or they could take a foe strategy in which no cooperation is needed for both strains’ survival. |
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