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Purdue/11 July 2008
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| + | ===Glycerol Stocks!=== | ||
| + | *Today making glycerol stocks of SOS (J22106) and LacZ (I732017) parts from stabs sent by iGEM | ||
| + | *Following iGEM protocol: | ||
| + | **Make o/n culture in antibiotic (amp) LB broth --did this yesterday | ||
| + | **Combine 1mL of overnight w/ 150 uL of sterile 80% glycerol solution in screw-top cryotube (make sure tube is labeled with part #, plasmid, and antibiotic resistance) | ||
| + | **Vortex briefly | ||
| + | **Incubate at room temp. for 1/2 hour and put in -80C freezer | ||
| + | |||
| + | In other news, the bead plating did not help the other Lac transformations to work, although the pUC19 still grew quite well. | ||
| + | |||
| + | '''Edited by Janie Stine''' | ||
Revision as of 16:38, 11 July 2008
Click Here to return to the notebook.
Glycerol Stocks!
- Today making glycerol stocks of SOS (J22106) and LacZ (I732017) parts from stabs sent by iGEM
- Following iGEM protocol:
- Make o/n culture in antibiotic (amp) LB broth --did this yesterday
- Combine 1mL of overnight w/ 150 uL of sterile 80% glycerol solution in screw-top cryotube (make sure tube is labeled with part #, plasmid, and antibiotic resistance)
- Vortex briefly
- Incubate at room temp. for 1/2 hour and put in -80C freezer
In other news, the bead plating did not help the other Lac transformations to work, although the pUC19 still grew quite well.
Edited by Janie Stine
