"
Purdue/2 September 2008
From 2008.igem.org
(Difference between revisions)
(New page: Click Here to return to the notebook.) |
|||
| Line 1: | Line 1: | ||
[[Team:Purdue/Notebook | Click Here to return to the notebook.]] | [[Team:Purdue/Notebook | Click Here to return to the notebook.]] | ||
| + | |||
| + | '''Restriction Digest of SOS and LacZ''' | ||
| + | |||
| + | 1. Mixed digestion solution: | ||
| + | *50uL DNA | ||
| + | *7uL NEB2 | ||
| + | *10.5uL ddH2O | ||
| + | *0.5uL BSA | ||
| + | *1uL of each enzyme | ||
| + | **SOS: EcoRI + SpeI | ||
| + | **LacZ: EcoRI + XbaI | ||
| + | **This makes SOS the small part cut out (192 bp) and put into the LacZ plasmid (~5000 bp...=LacZ + most of plasmid) | ||
| + | |||
| + | 2. Put mixtures at 37C for 1 hour | ||
| + | |||
| + | 3. Inactivated by..... | ||
| + | |||
| + | 4. Run through Agarose gel w/ DNA ladder | ||
| + | |||
| + | 5. Overnight stain w/ Fast Blast | ||
| + | |||
| + | |||
| + | '''Edited by Janie Stine''' | ||
Revision as of 15:25, 2 September 2008
Click Here to return to the notebook.
Restriction Digest of SOS and LacZ
1. Mixed digestion solution:
- 50uL DNA
- 7uL NEB2
- 10.5uL ddH2O
- 0.5uL BSA
- 1uL of each enzyme
- SOS: EcoRI + SpeI
- LacZ: EcoRI + XbaI
- This makes SOS the small part cut out (192 bp) and put into the LacZ plasmid (~5000 bp...=LacZ + most of plasmid)
2. Put mixtures at 37C for 1 hour
3. Inactivated by.....
4. Run through Agarose gel w/ DNA ladder
5. Overnight stain w/ Fast Blast
Edited by Janie Stine
