http://2008.igem.org/wiki/index.php?title=SOC&feed=atom&action=historySOC - Revision history2024-03-29T15:16:56ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=SOC&diff=3265&oldid=prevMargaretRuzicka: add SOC recipe2008-05-30T02:24:29Z<p>add SOC recipe</p>
<p><b>New page</b></p><div>== Summary ==<br />
SOC Medium. Used in [[Bacterial Transformation]].<br />
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== Ingredients ==<br />
*[[SOB]]<br />
*20 mM glucose<br />
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==Protocol==<br />
#Follow directions to make 1 liter of [[SOB]] media<br />
#After cooling medium to less than 50&deg;C, add 20 ml filter sterilized 20% glucose solution<br />
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==Source==<br />
Adapted From:<br />
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F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36<br />
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==Trivia==<br />
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.<br />
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[[Category:Material]] [[Category:Standard Media]]</div>MargaretRuzicka