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Combined display of all available logs of 2008.igem.org. You can narrow down the view by selecting a log type, the user name (case-sensitive), or the affected page (also case-sensitive).- 17:07, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin - presentation 01.10.08.ppt"
- 17:04, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 20-histo.png"
- 16:58, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin - part presentation 19.09.08.ppt"
- 16:47, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 19-081028-GFP eucaryotic.png"
- 16:42, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Status report 2008-09-09-colicins.ppt"
- 16:39, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin 13.08.08.ppt"
- 02:48, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Convert 2 wikitable.zip"
- 02:47, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Tecan scripts.zip"
- 02:41, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Timerow maxi.m"
- 02:41, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Timerow.m"
- 02:41, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:One plate.m"
- 02:40, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Sendertest.m"
- 02:40, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin char.m"
- 02:39, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin activity system lysis.m"
- 02:39, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin activity system.m"
- 02:39, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin one plate.m"
- 02:39, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin test.m"
- 02:39, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:One plate colicin.m"
- 02:32, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Colicin activity 081020.m" (Matlab script for colicin activity test data evaluation)
- 02:05, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 18-081028-PI eucaryotic.png" (Positive PI MCF-7 cells (%) over time. E coli producing colicin E1 protein cause cell death at the earliest time point (2 h) and keep it higher then the other condition. After 3 h, the reference strain also starts to present PI positive cells that increas)
- 02:04, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 17-081025-killerpreytest GFP 1.png" (Prey-Killer system test. GFP intensity of prey strains was measured over 12 h at 37 °C. Prey cells were killed for prey-killer ratios from 1:1 up to 25:1 (blue, green, red, light blue) due to the colicin production of the killer strain. This production w)
- 02:03, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 16-081028-lysis combined.png" (Visualization of prey-killer system. The prey (GFP) and the killer (mCherry) strain are mixed on a 0.5 % agarosepad. Killer cells were induced with AHL before adding to the medium. The killer cell on the left picture (white arrow) is vanished after 30 min)
- 02:02, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded a new version of "File:Fig 15-081027-Lysis.png" (Lysis test with LuxR-ColicinE1-Receiver dependent on AHL concentrations. On the left panel the growth of a killer population induced with different concentrations of AHL is shown. At the right panel the data of reference experiments are plotted. For AHL c)
- 02:02, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 15-081027-Lysis.png"
- 02:02, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 14-081023-bar diagram ratios 3conc.png" (Toxicity of LuxR-colicinE1receiver part for different prey-killer ratios. The graphs show GFP intensities of prey cells at t = 0 h and t = 12 h. For prey killer ratios of 1:1 all preys were killed after 12 hours even if there is no AHL present. For prey-k)
- 02:01, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 13-081023-dose responsecurve 100-1.png" (Dose-response curve of AHL concentration and killing efficiency of the killer strain for an prey-killer ratio of 100:1. The GFP intensities at t = 12 h are plotted against AHL concentrations (0 M – 1 nM). For AHL concentrations higher than 600 nM all pr)
- 02:01, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 12-081023-P-K 100-1 growth curve.png" (Results of colicin E1 toxicity tests for different AHL concentrations with a prey-killer ratio of 100:1. Top left panel: GFP intensities of prey cells treated with killer cells. Top right panel: Optical densities of prey cells treated with killer cells. )
- 02:01, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 11-081028-PCR control digestion.png" (Control digestion of the final pSB1A2-receiver-colicinE1 plasmid (LuxR-colicinE1-receiver). Bright bands of ladder from bottom to top: 3000 bp, 1000 bp and 500 bp. First two bands contain approximately 5200 bp, which is the whole plasmid, cutted once by X)
- 02:00, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 10-081028-PCR amplification colicins.png" (PCR amplification of colicin E1 operon, the receiver part insert (T9002 without GFP) ~1088 bp and the vector pSB1A2. )
- 01:57, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 9-PCR-amplification results cloning.png" (PCR-amplification results of the Colicin E9 short (~1050 bp) and long (~2014 bp) insert, the receiver part insert (T9002 without GFP) ~1088 bp and the vector pSB1A2. )
- 01:57, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 8-11092008-igem colicin activity sonicbath on GFP 3-1 beschriftet.png" (Colicin toxicity test of lysed supernatant on E. coli TOP 10 cells harbouring BBa_I20260: The GFP intensity is related to the growth of the E. coli TOP 10 cells. These could not grew while adding supernatant of the killing strain, but grew only with super)
- 01:54, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 6-081019-sendertestresults.png" (Results of sender activity test. Top left: Growth curve of sender culture. Top right: GFP/OD values of sender supernatants at t = 10 h. Bottom left: GFP/OD – AHL calibration curve. Dotted line visualized regression of an exponential growth curve. Botto)
- 01:49, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 7-081028-sender test GFP-OD.png" (GFP/OD results of sender activity measurements. On The left panel the GFP/OD values for induction with sender supernatants are shown and on the right panel the GFP/OD values for predefined AHL inductions can be observed. During the first sic hours the val)
- 01:48, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 6-081019-GFP-OD combined.png" (Results of sender activity test. Top left: Growth curve of sender culture. Top right: GFP/OD values of sender supernatants at t = 10 h. Bottom left: GFP/OD – AHL calibration curve. Dotted line visualized regression of an exponential growth curve. Botto)
- 01:47, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 5-081028-controldigestion sender.png" (Control digestion of the prey cell cloning (BBa_J23107+BBa_F1610). Bright bands of the DNA ladder from bottom to top: 3000 bp, 1000 bp and 500 bp. Expected bands for the digestion: ~850 bp for the Insert (BBa_J23107+BBa_F1610) and ~2070 bp for the vector )
- 01:42, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded a new version of "File:Fig 4-cloningstrategy colicinreceivers small.png" (Cloning strategy of the LuxR-colicin-receiver parts (E1: BBa_K150009, E9 long: BBa_K150011, E9 short: BBa_K150013) First of all the LuxR receiver part (BBa_T9002) was amplified by PCR. The product, containing a Ptet promoter, the luxR gene followed by the)
- 01:41, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 4-cloningstrategy colicinreceivers small.png" (Cloning strategy of the LuxR-colicin-receiver parts (E1: BBa_K150009, E9 long: BBa_K150011, E9 short: BBa_K150013) First of all the LuxR receiver part (BBa_T9002) was amplified by PCR. The product, containing a Ptet promoter, the luxR gene followed by the)
- 01:30, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 4-cloningstrategy colicinreceivers.png" (Cloning strategy of the LuxR-colicin-receiver parts (E1: BBa_K150009, E9 long: BBa_K150011, E9 short: BBa_K150013) First of all the LuxR receiver part (BBa_T9002) was amplified by PCR. The product, containing a Ptet promoter, the luxR gene followed by the)
- 01:30, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 3-cloningstrategy sender.png" (Cloning strategy of the Sender part (BBa_K15000) Parts BBa_J23107 and BBa_F1610 were combined according to the BioBrickTM assembly. Therefore BBa_J23107 on BBa_J61002 was digested with SpeI and PstI and BBa_F1610 was digested with XbaI and PstI. Cloning w)
- 01:28, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:HD-Fig 2-colicinE1 structure.png" ((a) Ribbon diagram of the Colicin E1 pore-forming domain by X ray crystal structure. The channel forming domain contains 10 helices (I-X). The two hydrophobic helices (VIII and IX) are labeled yellow. (b) Colicin E1 in a membrane bound state in two perspe)
- 01:27, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded a new version of "File:HD-Fig 1-gene organization colicin.png" (Organization of colicin genes. Genes on the leading strand are represented by black arrows, gene on the opposite strand by blue arrow. SOS promoter (PSOS), immunity promoter (Pim) and terminators (T) are indicated by arrows. The first gene in each colicin)
- 01:27, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:HD-Fig 1-gene organization colicin.png"
- 01:24, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded a new version of "File:Fig 0-System.png" (Scheme of Colicin System. The prey contains genes for LuxI which produces AHL. The killer cells are able to sense AHL by LuxR which activates the colicin production and release.)
- 01:22, 29 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:Fig 0-System.png"
- 01:12, 28 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:081021-controldigestion.jpg"
- 21:55, 25 October 2008 Andreaskuehne (Talk | contribs) uploaded "File:080813 gel labeled digestion small.jpg"
