User contributions
From 2008.igem.org
- 23:11, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 23:11, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 23:11, 29 October 2008 (diff | hist) N File:MelbourneTeam.jpg (top)
- 14:27, 29 October 2008 (diff | hist) Team:Melbourne/Components (top)
- 14:27, 29 October 2008 (diff | hist) Template:MelbournePanel (top)
- 14:17, 29 October 2008 (diff | hist) Team:Melbourne/LinModel (top)
- 14:17, 29 October 2008 (diff | hist) Team:Melbourne/BinModel (top)
- 14:16, 29 October 2008 (diff | hist) Team:Melbourne/LabNote (top)
- 14:16, 29 October 2008 (diff | hist) Team:Melbourne/Products (top)
- 14:16, 29 October 2008 (diff | hist) Team:Melbourne/Components
- 14:16, 29 October 2008 (diff | hist) Team:Melbourne/Overview (top)
- 14:15, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 14:15, 29 October 2008 (diff | hist) Team:Melbourne (top)
- 14:12, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 14:06, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 14:03, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 14:02, 29 October 2008 (diff | hist) N File:BinaryClock.pdf (top)
- 13:55, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:53, 29 October 2008 (diff | hist) Team:Melbourne/LinModel
- 13:53, 29 October 2008 (diff | hist) Team:Melbourne/BinModel
- 13:52, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/Components
- 13:50, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 13:49, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:48, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:47, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 12:56, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 12:55, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 12:55, 29 October 2008 (diff | hist) N Melbourne/5 August 2008 (New page: ==Done today:== * Red light retest. 4 transformations: *Cph8 + pPL-PCB *Cph8 *PCB *-ve control Protocol: *2μL DNA *RU1012 *30 min ice *1 min 42°C *1 hour at 37°C + LB Each transfor...) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/3 August 2008 (New page: ==Done today:== * Inoculated cultures with colonies from yesterday's transformations.) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/2 August 2008 (New page: ==Done today:== * Transformed the 3 ligations set up yesterday into DH5alpha cells. Plated on Amp.) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/1 August 2008 (New page: ==Done today:== * 3 Ligations left for overnight incubation: **pGlO with lock 3d (P2 8H) **pGLO with lock 3b (P2 8F) **pGLO with P8 1C (empty vector)) (top)
- 12:52, 29 October 2008 (diff | hist) N Melbourne/31 July 2008 (New page: ==Done today:== * Purified PCR products of pGLO (from mp 2, 3, 4) * Restriction digests left for overnight incubation: ** pGLO with X, P ** P8 1C with S, P ** P2 8F (lock 3b) with S, P **...) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/25 July 2008 (New page: ==Done today:== * Miniprepped: ** pGLO (again) as backup ** P0 3A ** P0 5A ** P14 8E ** P8 1C) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/24 July 2008 (New page: ==Done today:== * No growth from P13 7F or P3 4A from yesterday * Cultured successful transformations in appropriate antibiotic) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/23 July 2008 (New page: ==Done today:== * Transformed ** P13 7F (FBL) ** P14 8E (FBL) ** P0 5A (FBL) ** P0 3A (FBL) ** P3 4A (RS) See Biobrick section for w...) (top)
- 12:50, 29 October 2008 (diff | hist) N Melbourne/22 July 2008 (New page: ==Done today:== * Suspended and transformed P8 1C & P3 4A (empty Amp vectors). * Miniprepped pGLO.) (top)
- 12:49, 29 October 2008 (diff | hist) N Melbourne/18 July 2008 (New page: ==Done today:== * Plated the red light double transformation. Left to incubate on lab bench--one wrapped in foil, the other in glad wrap.) (top)
- 12:48, 29 October 2008 (diff | hist) N Melbourne/17 July 2008 (New page: ==Done today:== * Re-transformed P22 6E (RL), P13 4F, 8A (FBL)--plated on Kan+IPTG * Double transformation of pLPCB & CPH8 into RU1012 cells--to be plated tomorrow on Amp+Kan+Chlor+IPTG+X-...) (top)
- 12:48, 29 October 2008 (diff | hist) N Melbourne/15 July 2008 (New page: ==Done today:== * Miniprepped PO 3F, 3E, 4C, 4G * Set up cultures P1 7E, P13 9G) (top)
- 12:48, 29 October 2008 (diff | hist) N Melbourne/14 July 2008 (New page: ==Done today:== * Re-transformed: ** P13 4F, 8A (FBL) ** P22 6E, P13 9G (RL) ** P1 7E (RS) ''Result:'' Only P13 9G and P1 7E grew. * Made Kan plates) (top)
- 12:47, 29 October 2008 (diff | hist) N Melbourne/11 July 2008 (New page: ==Done today:== * Miniprepped P13 9H, P6 3D, P2 9A, P13 5E, P13 5C) (top)
- 12:46, 29 October 2008 (diff | hist) N Melbourne/9 July 2008 (New page: ==Done today:== * Re-transformed P13 9H (RL), P6 3D (RS) * Suspended and transformed P2 9A (RS) Used slightly modified protocol. All plates showed growth.) (top)
- 12:46, 29 October 2008 (diff | hist) N Melbourne/8 July 2008 (New page: ==Done today:== * Retransformed : P13 4F, 8A (FBL) P13 9G, P13 9H, P22 6E (RL) Also set up a positive control (pET 30-8)--Kanamycin resistant vector * Suspended and transformed P6 3...) (top)
- 12:45, 29 October 2008 (diff | hist) N Melbourne/3 July 2008 (New page: ==Done today:== * Miniprepped: ** P0 C4, E3, F3, G4 (FBL) ** P3 8C, P1 12F (RS) * Made glycerol stocks * Transformed: ** P22 8C, 6E ** P16 5F ** P13 5E, 9G ** P2 5E ** P0 2E * Punched:...) (top)
- 12:44, 29 October 2008 (diff | hist) N Melbourne/1 July 2008 (New page: ==Done today:== * Suspended from Kit {| border = "0.5" |- ! Part ! Plate ! Well |- | M30109 | 1022 | 8C |- | I15010 | 1022 | 6E |- | I15008 | 1013 | 9G |- | I15009 | 1013 | 5E |- | R00...) (top)
- 12:43, 29 October 2008 (diff | hist) N Melbourne/30 June 2008 (New page: ==Done today:== * Made competent DH5alpha cells.) (top)
- 12:43, 29 October 2008 (diff | hist) N Melbourne/1 June 2008 (New page: ==Done today:== * Suspension and transformation (of DH5alpha cells) with P1003 8C (J36846 pTet mRFP)...for ribolock as reporter P1002 8A (I13522 pTet GFP) P1002 3H (I13600 pTet CFP) ...) (top)
- 12:41, 29 October 2008 (diff | hist) N Melbourne/20 May 2008 (New page: ==Done today:== *Preparation of pL-pCB and pCph8 for sequencing ** 100μM primer stock stored in red light box in -20°C freezer *O/N culture of pL-pCB and pCph8 for miniprep tomorrow ...) (top)
- 12:40, 29 October 2008 (diff | hist) N Melbourne/13 May 2008 (New page: ==Done today:== ''Glycerol stocks'' made from overnight cultures (see yesterday for details) *800μL culture *600μL 100% glycerol Stored in -...) (top)
- 12:39, 29 October 2008 (diff | hist) N Melbourne/12 May 2008 (New page: ==Done today:== Transformations and overnight culture for glycerol stocks- Three cultures: *pCph8 in RU1012 cells *pL-pCB in RU1012 cells *pCph8/pL-pCB in RU1012 cells) (top)
- 12:38, 29 October 2008 (diff | hist) N Melbourne/5 May 2008 (New page: ==Done today:== Transformation for glycerol stocks: *RU1012 cells *1 μL DNA *Three transformations: **pCph8 **pL-PCB **pCph8/pL-PCB *Standard transformation protocol) (top)
- 12:37, 29 October 2008 (diff | hist) N Melbourne/16 April 2008 (New page: ==Done today:== Red light test (cont): Unfortunately both plates turned blue. This was most likely because the control was not in the light all the time. The pPL-PCB plasmid was not indu...) (top)
- 12:36, 29 October 2008 (diff | hist) N Melbourne/15 April 2008 (New page: ==Done today:== Red light test *Double transformation that Alisa did was used as folows: *2 plates (Amp/Kan/Chlor) had Xgal added (20 mg/mL) by disolving 1.6 mg of Xgal in 80 μL of DMSO ...) (top)
- 12:24, 29 October 2008 (diff | hist) N Melbourne/11 April 2008 (New page: ==Done today:== * Ru1012 cells are competent (less effient than DH5α)) (top)
- 12:24, 29 October 2008 (diff | hist) N Melbourne/10 April 2008 (New page: ==Done today:== RU1012 cells were tested for competency *1 μL of P48J test plamid used *30 min on ice *1 min heat shock at 42°C *Alisa plated samples on Amp plates) (top)
- 12:22, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 12:19, 29 October 2008 (diff | hist) N Melbourne/4 April 2008 (New page: ==Done today== *Mini-Prep of two CPH8 cultures and two pL-pCB cultures *Stored in iGEM freezer) (top)
- 12:13, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 11:55, 29 October 2008 (diff | hist) N Team:Melbourne/LabNote (New page: <table> <html> <style type="text/css"> table.calendar { margin: 0; padding: 2px; } table.calendar td { margin: 0; padding: 1px; vertical-align: top; } table.month .heading ...)
- 11:41, 29 October 2008 (diff | hist) Template:MelbournePanel
- 11:11, 29 October 2008 (diff | hist) Team:Melbourne/Architecture (Removing all content from page) (top)
- 11:11, 29 October 2008 (diff | hist) Template:MelbournePanel
- 11:10, 29 October 2008 (diff | hist) Template:MelbournePanel
- 11:08, 29 October 2008 (diff | hist) Team:Melbourne/BinModel
- 11:08, 29 October 2008 (diff | hist) N File:Bin model.jpg (top)
- 11:06, 29 October 2008 (diff | hist) Team:Melbourne/LinModel
- 01:26, 29 October 2008 (diff | hist) Jamboree/Schedule/Practice sessions (→Friday November 7 : Practice Talks sign-up sheet)
- 21:58, 28 October 2008 (diff | hist) Team:Melbourne/Products
- 21:58, 28 October 2008 (diff | hist) Team:Melbourne/Components
- 21:57, 28 October 2008 (diff | hist) N Team:Melbourne/Components (New page: * Riboswitch * Red light sensor * Positive feedback loop)
- 21:55, 28 October 2008 (diff | hist) Team:Melbourne (Replacing page with '{{MelbourneHeader}} {{MelbournePanel}} <div id="mainpage"> The River of Meditation Winter [[Im...')
- 21:54, 28 October 2008 (diff | hist) N File:Bio21Building.jpg (top)
- 21:47, 28 October 2008 (diff | hist) N File:MUTree.jpg (top)
- 21:47, 28 October 2008 (diff | hist) N File:MUWater.jpg (top)
- 11:17, 28 October 2008 (diff | hist) Team:Melbourne/Overview
- 10:27, 28 October 2008 (diff | hist) Team:Melbourne/Team
- 10:23, 28 October 2008 (diff | hist) Team:Melbourne/Team
- 10:20, 28 October 2008 (diff | hist) File:MelbourneTeamLogo.jpg (uploaded a new version of "Image:MelbourneTeamLogo.jpg") (top)
- 10:19, 28 October 2008 (diff | hist) N File:MelbourneTeamLogo.jpg
- 10:18, 28 October 2008 (diff | hist) Team:Melbourne/Team
- 10:17, 28 October 2008 (diff | hist) Team:Melbourne/Products
- 10:16, 28 October 2008 (diff | hist) Team:Melbourne/Products
- 11:59, 27 October 2008 (diff | hist) Team:Melbourne/Overview
- 11:58, 27 October 2008 (diff | hist) Team:Melbourne/Overview
- 11:41, 27 October 2008 (diff | hist) Team:Melbourne/LinModel (→Extension)
- 11:41, 27 October 2008 (diff | hist) Team:Melbourne/LinModel (→==)
- 11:41, 27 October 2008 (diff | hist) Team:Melbourne/LinModel (→Extension)
- 11:40, 27 October 2008 (diff | hist) Team:Melbourne/LinModel (→Extension)
- 11:40, 27 October 2008 (diff | hist) Team:Melbourne/LinModel
- 11:38, 27 October 2008 (diff | hist) Team:Melbourne/LinModel
- 11:36, 27 October 2008 (diff | hist) Team:Melbourne/Architecture
- 11:36, 27 October 2008 (diff | hist) Team:Melbourne/Architecture
- 11:35, 27 October 2008 (diff | hist) Team:Melbourne/Overview
- 11:35, 27 October 2008 (diff | hist) Team:Melbourne/Overview
- 11:34, 27 October 2008 (diff | hist) Team:Melbourne/Overview
- 11:33, 27 October 2008 (diff | hist) Template:MelbourneHeader (top)
- 11:31, 27 October 2008 (diff | hist) Template:MelbourneHeader
- 11:30, 27 October 2008 (diff | hist) Team:Melbourne/Team
- 11:26, 27 October 2008 (diff | hist) Team:Melbourne/Team
- 11:24, 27 October 2008 (diff | hist) N Template:MelbournePanel (New page: {| style="color:dodgerblue;background-color:#21aa55;" cellpadding="3" cellspacing="1" border="1" bordercolor="dodgerblue" width="85%" align="center" !align="center"|[[Team:Melbourne|<font...)
- 11:24, 27 October 2008 (diff | hist) Team:Melbourne
- 11:23, 27 October 2008 (diff | hist) Template:MelbourneHeader
- 11:08, 27 October 2008 (diff | hist) Team:Melbourne/Team
- 10:20, 27 October 2008 (diff | hist) Team:Melbourne/Team
- 10:02, 27 October 2008 (diff | hist) Team:Melbourne
- 09:20, 27 October 2008 (diff | hist) N File:CityOfMelbourne.jpg (top)
- 09:20, 27 October 2008 (diff | hist) Wiki/Template:MelbourneHeader (top)
- 03:34, 27 October 2008 (diff | hist) Team:Melbourne/Products
- 03:34, 27 October 2008 (diff | hist) N Team:Melbourne/Products (New page: *'''Riboswitch''' ** '''Parts Designed''' *** lacI promoter + Key3C *** pTet->lock3d->GFP->Ter **'''Parts Constructed''' *** lacI promoter + Key3C)
- 11:58, 26 October 2008 (diff | hist) Team:Melbourne/Architecture
- 11:56, 26 October 2008 (diff | hist) Template:MelbourneHeader
- 11:55, 26 October 2008 (diff | hist) Team:Melbourne/Overview
- 11:54, 26 October 2008 (diff | hist) Template:MelbourneHeader
- 11:51, 26 October 2008 (diff | hist) Template:MelbourneHeader
- 11:47, 26 October 2008 (diff | hist) Team:Melbourne
- 11:47, 26 October 2008 (diff | hist) Team:Melbourne
- 11:47, 26 October 2008 (diff | hist) N Template:MelbourneHeader (New page: {| | |<p style = "font-size:400%;">Melbourne iGEM 2008</p> Image:Melbourne uni logo.jpg Image:Bio21 institute.jpg |} <...)
- 11:41, 26 October 2008 (diff | hist) Team:Melbourne
- 11:40, 26 October 2008 (diff | hist) Team:Melbourne
- 11:38, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:37, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:34, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:33, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:31, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:31, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:30, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:30, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:21, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:20, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:20, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:18, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:17, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:16, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:15, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:15, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:14, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:06, 26 October 2008 (diff | hist) Wiki/Template:MelbourneHeader
- 11:01, 26 October 2008 (diff | hist) N Wiki/Template:MelbourneHeader (New page: {| | |<p style = "font-size:255%;">Melbourne iGEM 2008</p> Image:Melbourne uni logo.jpg Image:Bio21 institute.jpg |} </...)
- 10:35, 26 October 2008 (diff | hist) Team:Melbourne
- 10:35, 26 October 2008 (diff | hist) Team:Melbourne
- 10:24, 26 October 2008 (diff | hist) Team:Melbourne
- 10:23, 26 October 2008 (diff | hist) Team:Melbourne
- 10:20, 26 October 2008 (diff | hist) Team:Melbourne
- 10:20, 26 October 2008 (diff | hist) Team:Melbourne
- 10:17, 26 October 2008 (diff | hist) Team:Melbourne
- 10:02, 26 October 2008 (diff | hist) Team:Melbourne
- 10:01, 26 October 2008 (diff | hist) Team:Melbourne
- 10:00, 26 October 2008 (diff | hist) N File:Bio21 institute.jpg (top)
- 09:58, 26 October 2008 (diff | hist) Team:Melbourne
- 09:51, 26 October 2008 (diff | hist) Team:Melbourne
- 09:49, 26 October 2008 (diff | hist) File:Melbourne uni logo.jpg (uploaded a new version of "Image:Melbourne uni logo.jpg") (top)
- 09:43, 26 October 2008 (diff | hist) N File:Melbourne uni logo.jpg
- 09:16, 26 October 2008 (diff | hist) N File:Simon model.jpg (top)
- 09:16, 26 October 2008 (diff | hist) N Team:Melbourne/BinModel (New page: Return to Melbourne Homepage Image:Simon_model.jpg)
- 09:14, 26 October 2008 (diff | hist) Team:Melbourne/LinModel
- 09:13, 26 October 2008 (diff | hist) N File:BCSteadyState2.jpg (top)
- 09:12, 26 October 2008 (diff | hist) N File:BCSecondPulse.jpg (top)
- 09:11, 26 October 2008 (diff | hist) N File:BCSteadyState1.jpg (top)
- 09:10, 26 October 2008 (diff | hist) N File:BCFirstPulse.jpg (top)
- 09:08, 26 October 2008 (diff | hist) N File:BCInit.jpg (top)
- 08:06, 26 October 2008 (diff | hist) N File:BCFull.jpg (top)
- 08:01, 26 October 2008 (diff | hist) N Team:Melbourne/LinModel (New page: Return to Melbourne Homepage Return to Architecture [[IGEM:Melbourne/2008/BCModelling/Different Models of the BioClock|Retu...)
- 07:58, 26 October 2008 (diff | hist) N Team:Melbourne/Architecture (New page: == Linear Model== == Binary Model==)
- 07:53, 26 October 2008 (diff | hist) Team:Melbourne
- 07:48, 26 October 2008 (diff | hist) N Team:Melbourne/Overview (New page: Return to Melbourne Homepage The original binary idea. The ultimate goal of this idea is to construct a biological pro...)
- 07:45, 26 October 2008 (diff | hist) N Team:Melbourne/Test (New page: Yes, here) (top)
- 07:45, 26 October 2008 (diff | hist) Team:Melbourne
- 07:42, 26 October 2008 (diff | hist) Team:Melbourne
- 07:40, 26 October 2008 (diff | hist) Team:Melbourne
- 07:19, 26 October 2008 (diff | hist) N /BCArchitecture (New page: Return to Melbourne Homepage Return to Architecture [[IGEM:Melbourne/2008/BCModelling/Different Models of the BioClock|Retu...) (top)
- 07:17, 26 October 2008 (diff | hist) /BCOverview (top)
- 07:16, 26 October 2008 (diff | hist) N /BCOverview (New page: Return to Melbourne Homepage The original binary idea of the bioclock was proposed by Di. The ultimate goal of th...)
- 07:12, 26 October 2008 (diff | hist) Team:Melbourne
- 05:40, 25 October 2008 (diff | hist) Team:Melbourne
- 07:51, 10 August 2008 (diff | hist) Team:Melbourne
- 07:50, 10 August 2008 (diff | hist) Team:Melbourne (Replacing page with ''''Welcome to Melbourne iGEM 2008 Team!''' [http://www.openwetware.org/wiki/IGEM:Melbourne/2008 Melbourne iGEM 2008 Team Wiki]')