User contributions
From 2008.igem.org
(Latest | Earliest) View (newer 20 | older 20) (20 | 50 | 100 | 250 | 500)
- 08:30, 4 October 2008 (diff | hist) N Beijing Normal/31 July 2008 (New page: Today we discussed about the bottleneck step in the PCBs degradation pathway. DHBD(2,3-dihydroxybiphenyl 1,2-dioxygenase), the third enzyme of the bph pathway, is of particular significan...) (top)
- 08:28, 4 October 2008 (diff | hist) N Beijing Normal/30 July 2008 (New page: Today is Sally's birthday. We met up in the morning to discuss the design. And we have a break in the afternoon.) (top)
- 08:26, 4 October 2008 (diff | hist) N Beijing Normal/29 July 2008 (New page: We have dicussion on all the referneces we have read these days. And several ideas are proposed. We plan to devide the whole project into two parts: PCBs degradation effeciency enhancing a...) (top)
- 08:22, 4 October 2008 (diff | hist) N Beijing Normal/28 July 2008 (New page: The U.S.A. Olympic team arrived at our university today. We are all very excited. There are so many super stars here now. I hope I can see Kobe Bryant somewhere~~ But also comes the bad n...) (top)
- 08:14, 4 October 2008 (diff | hist) N Beijing Normal/23 July 2008 (New page: All the primers are designed according to the standard.) (top)
- 08:12, 4 October 2008 (diff | hist) N Beijing Normal/22 July 2008 (New page: We go on to transform the pSB1AC3 plasmids.) (top)
- 08:10, 4 October 2008 (diff | hist) Beijing Normal/18 July 2008 (top)
- 08:10, 4 October 2008 (diff | hist) N Beijing Normal/22 September 2008 (New page: We discussed about the three sensor systems in our project together. rhyB and sodB system were determined to be used. dxnA, redA2 were amplified from the SW1 strain.) (top)
- 08:06, 4 October 2008 (diff | hist) N Beijing Normal/23 September 2008 (New page: We had a discussion about the final version of our design. And Stephen tought us to perform the site mutation experiment.)
- 08:03, 4 October 2008 (diff | hist) N Beijing Normal/24 September 2008 (New page: The plasmids pAIA73 is reansformed into Top10. The plasmids will be extracted and PCR will be performed.) (top)
- 08:03, 4 October 2008 (diff | hist) N Beijing Normal/25 September 2008 (New page: The plasmids pAIA73 is extracted. And we amplify the genes bphA1A2A3A4, bphB, bphC and bphD from this template. The yield of PCR is pretty good except bphA1A2A3A4. Maybe because it is a bi...) (top)
- 07:58, 4 October 2008 (diff | hist) N Beijing Normal/26 September 2008 (New page: All the genes are surveyed and all the digestion sites are determined.) (top)
- 07:54, 4 October 2008 (diff | hist) N Beijing Normal/4 October 2008 (New page: Site mutation of dxnA and dbfB failed. We suspect that the concentrations of the two fragment are far different so the annealing step has an unsatisfying yield. We perform the same experim...) (top)
- 11:26, 3 October 2008 (diff | hist) N Beijing Normal/26 July 2008 (New page: The primers of pSB1AC3 are designed. THe vector will be amplyfied for futher use.) (top)
- 11:23, 3 October 2008 (diff | hist) N Beijing Normal/27 September 2008 (New page: The three sensor systems are reexamined. The induce concentration is to be confirmed.) (top)
- 11:21, 3 October 2008 (diff | hist) N Beijing Normal/28 September 2008 (New page: We restudy the deisgn again and several suggestion are proposed.) (top)
- 11:20, 3 October 2008 (diff | hist) N Beijing Normal/29 September 2008 (New page: fdx3 is amplifyed and following protocols are performed but the transformation filed. We suspect that the restrition digestion step is not long enough.) (top)
- 11:18, 3 October 2008 (diff | hist) N Beijing Normal/30 September 2008 (New page: we held a meeting to discuss about the references.) (top)
- 11:13, 3 October 2008 (diff | hist) N Beijing Normal/1 October 2008 (New page: construction of dxnA and dxnB is performed. But failed.) (top)
- 11:12, 3 October 2008 (diff | hist) N Beijing Normal/2 October 2008 (New page: Today we amplify the genes of bphB, bphC and bphD. The yield is pretty satisfying. Then restriction digestion is performed for three hours and ligation is performed. After transformation ...) (top)
(Latest | Earliest) View (newer 20 | older 20) (20 | 50 | 100 | 250 | 500)