User contributions
From 2008.igem.org
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- 13:52, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/Components
- 13:50, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 13:49, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:48, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:47, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 12:56, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 12:55, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 12:55, 29 October 2008 (diff | hist) N Melbourne/5 August 2008 (New page: ==Done today:== * Red light retest. 4 transformations: *Cph8 + pPL-PCB *Cph8 *PCB *-ve control Protocol: *2μL DNA *RU1012 *30 min ice *1 min 42°C *1 hour at 37°C + LB Each transfor...) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/3 August 2008 (New page: ==Done today:== * Inoculated cultures with colonies from yesterday's transformations.) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/2 August 2008 (New page: ==Done today:== * Transformed the 3 ligations set up yesterday into DH5alpha cells. Plated on Amp.) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/1 August 2008 (New page: ==Done today:== * 3 Ligations left for overnight incubation: **pGlO with lock 3d (P2 8H) **pGLO with lock 3b (P2 8F) **pGLO with P8 1C (empty vector)) (top)
- 12:52, 29 October 2008 (diff | hist) N Melbourne/31 July 2008 (New page: ==Done today:== * Purified PCR products of pGLO (from mp 2, 3, 4) * Restriction digests left for overnight incubation: ** pGLO with X, P ** P8 1C with S, P ** P2 8F (lock 3b) with S, P **...) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/25 July 2008 (New page: ==Done today:== * Miniprepped: ** pGLO (again) as backup ** P0 3A ** P0 5A ** P14 8E ** P8 1C) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/24 July 2008 (New page: ==Done today:== * No growth from P13 7F or P3 4A from yesterday * Cultured successful transformations in appropriate antibiotic) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/23 July 2008 (New page: ==Done today:== * Transformed ** P13 7F (FBL) ** P14 8E (FBL) ** P0 5A (FBL) ** P0 3A (FBL) ** P3 4A (RS) See Biobrick section for w...) (top)
- 12:50, 29 October 2008 (diff | hist) N Melbourne/22 July 2008 (New page: ==Done today:== * Suspended and transformed P8 1C & P3 4A (empty Amp vectors). * Miniprepped pGLO.) (top)
- 12:49, 29 October 2008 (diff | hist) N Melbourne/18 July 2008 (New page: ==Done today:== * Plated the red light double transformation. Left to incubate on lab bench--one wrapped in foil, the other in glad wrap.) (top)
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