http://2008.igem.org/wiki/index.php?title=Special:Contributions/KP&feed=atom&limit=50&target=KP&year=&month=
2008.igem.org - User contributions [en]
2024-03-28T22:30:27Z
From 2008.igem.org
MediaWiki 1.16.5
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-10-16T14:45:47Z
<p>KP: /* Dry Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' reading, molecular gastronomy, architecture and making Fanny crazy''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br> [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br> [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://www.master.bmc.upmc.fr/ Master BMC] (Molecular and Cellular Biology, specialized in Biochemistry) <br> and [http://www.mastbio.ens.fr/ ENS] <br>'''Interests:''' music, languages<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br> [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' stinky cheese and thistle vine<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] <br> [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student <br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-10-16T14:45:02Z
<p>KP: /* Dry Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' reading, molecular gastronomy, architecture and making Fanny crazy''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br> [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br> [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://www.master.bmc.upmc.fr/ Master BMC] (Molecular and Cellular Biology, specialized in Biochemistry) <br> and [http://www.mastbio.ens.fr/ ENS] <br>'''Interests:''' music, languages<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br> [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' stinky cheese and thistle vine<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] Grad Student<br> [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student<br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-10-16T14:44:11Z
<p>KP: /* Wet Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' reading, molecular gastronomy, architecture and making Fanny crazy''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br> [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br> [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://www.master.bmc.upmc.fr/ Master BMC] (Molecular and Cellular Biology, specialized in Biochemistry) <br> and [http://www.mastbio.ens.fr/ ENS] <br>'''Interests:''' music, languages<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>08' Graduated for [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' stinky cheese and thistle vine<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] Grad Student<br>and 08' Graduated for [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student<br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-10-15T15:29:08Z
<p>KP: /* Wet Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' reading, molecular gastronomy, architecture and making Fanny crazy''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br>08' Graduated for [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://www.master.bmc.upmc.fr/ Master BMC] (Molecular and Cellular Biology, specialized in Biochemistry) <br> and [http://www.mastbio.ens.fr/ ENS] <br>'''Interests:''' music, languages<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>08' Graduated for [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' stinky cheese and thistle vine<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] Grad Student<br>and 08' Graduated for [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student<br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-10-15T15:27:32Z
<p>KP: /* Wet Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' reading, molecular gastronomy, architecture and making Fanny crazy''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br>08' Graduated for [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://biochimie.snv.jussieu.fr/master/master_accueil.htm/ Master BMC] (Molecular and Cellular Biology, specialized in Biochemistry) <br> and [http://www.mastbio.ens.fr/ ENS] <br>'''Interests:''' music, languages<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>08' Graduated for [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' stinky cheese and thistle vine<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] Grad Student<br>and 08' Graduated for [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student<br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-10-15T15:22:35Z
<p>KP: /* Wet Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' reading, molecular gastronomy, architecture and making Fanny crazy''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br>08' Graduated for [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://biochimie.snv.jussieu.fr/master/master_MCT.htm/ Master BMC] (Molecular and Cellular Biology, specialized in Biochemistry) <br> and [http://www.mastbio.ens.fr/ ENS] <br>'''Interests:''' music, languages<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>08' Graduated for [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' stinky cheese and thistle vine<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] Grad Student<br>and 08' Graduated for [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student<br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-10-15T15:21:00Z
<p>KP: /* Wet Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' reading, molecular gastronomy, architecture and making Fanny crazy''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br>08' Graduated for [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://biochimie.snv.jussieu.fr/master/master_MCT.htm/ Master BMC (Molecular and Cellular Biology, specialized in Biochemistry)] <br> and [http://www.mastbio.ens.fr/ ENS] <br>'''Interests:''' music, languages<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>08' Graduated for [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' stinky cheese and thistle vine<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] Grad Student<br>and 08' Graduated for [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student<br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-10-15T15:20:22Z
<p>KP: /* Wet Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' reading, molecular gastronomy, architecture and making Fanny crazy''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br>08' Graduated for [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> [http://biochimie.snv.jussieu.fr/master/master_MCT.htm/ Master BMC (Molecular and Cellular Biology, specialized in Biochemistry)] and [http://www.mastbio.ens.fr/ ENS] <br>'''Interests:''' music, languages<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>08' Graduated for [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' stinky cheese and thistle vine<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] Grad Student<br>and 08' Graduated for [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student<br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/Team:Paris/Team/Students
Team:Paris/Team/Students
2008-09-25T15:25:16Z
<p>KP: /* Wet Lab */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Wet Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Alexandra-Bouaziz.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Alexandra Bouaziz'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Philippe-Bouziz.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Philippe Bouaziz'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry<br />
| style="width:240px;height:20px;" align="left" | pharmacochemistry and physicochemistry (nanotechnology)<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.biochimie.univ-paris7.fr/master/home.html Master SPGF] Undergrad Student <br>'''Interests:''' <br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.mms.master.univ-paris7.fr/web/ Master MMS] and [http://www.aiv-paris.org/fr/master-aiv/ Master AIV] Undergrad Student <br>'''Interests:''' <br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Guillaume.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Guillaume Bouchard'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Image_125.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Fanny Caffin'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Physics<br />
| style="width:240px;height:20px;" align="left" | Molecular and Cellular Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www2.ac-lyon.fr/etab/lycees/lyc-69/lyjperrin/index.html Lycée Jean-Perrin de Lyon] High School Student<br>Member of [http://www.scienceacademie.org/ Science Academie]<br>'''Interests:''' Astronomy<br />
| style="width:240px;" align="left" |'''Background:''' <br>08' Graduated for [http://master.univ-cancerologie.net/ Master Cancérologie]<br> '''Interests:''' Biology, but also drawing, photography, reading... and obviously : kick the butt of [https://2008.igem.org/Image:Philippe-Bouziz.jpg Philippe]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:N764862654 816423 9815.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Benoît d'Hayer'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Audrey-Desgrange.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Audrey Desgrange'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Genetics and Pharmacy<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br> [http://www.pharmacie.univ-paris5.fr/ Pharmacy] [http://www.univ-paris5.fr/ Paris Descartes]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' Oenology<br />
| style="width:240px;" align="left" | '''Background :''' <br>[http://www.ices.fr/ ICES] Sophomore Student<br> '''Interests:''' Dance, Chocolate, Physiology, Genetics<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Ana-Jimenez.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Ana Jimenez'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Cyprien.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''Cyprien Maisonnier'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
| style="width:240px;height:20px;" align="left" | Biology<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br> Biology [http://www.univ-paris-diderot.fr/ Paris Diderot]<br>and 08' Graduated for [http://www.univ-paris-diderot.fr/magisteregenet/ Master Européen de Génétique]<br> '''Interests:''' (Ana)<br />
| style="width:240px;" align="left" |'''Background:''' <br>[http://www.ens.fr/ Ecole Normale Supérieure] Undergrad Student ([http://www.biologie.ens.fr/ Molecular Biology and Genetics]) <br> '''Interests:''' Synthetic Biology, ''Saccharomyces cerevisiae'', ''Oenococcus oeni'' and ''Penicillium roqueforti''<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Photo 158.JPG| 130 px ]]<br />
| style="width:240px;" align="left" | '''Kok-Phen Yan'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Biology and Biochemistry<br />
|-<br />
| style="width:240px;" align="left" |'''Background:''' <br>08' Graduated for [http://www.master.bmc.upmc.fr/ Master BMC] <br>'''Interests:''' (Kok-Phen)<br />
|}<br />
<br><br />
<br />
=Dry Lab=<br />
<br />
<br><br />
{| align="center"<br />
|-<br />
<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Felipe-Golib.jpg|130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Felipe Golib'''<br />
| style="width:30px;" rowspan="3" |<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Louis-hedde.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Louis Hedde'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Mathematician<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>08' Graduated for [http://www.master-aiv.fr/ Master AIV] <br>'''Interests:''' Computer Science and Integrative Biology<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ensmp.fr/Accueil/ Ecole des Mines de Paris] Undergrad Student<br>'''Interests:''' (Louis)<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Yann-Lecunff.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Yann Le Cunff'''<br />
| style="width:30px;" rowspan="3" | <br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Hugo-Raguet.jpg| 130 px ]]<br />
| style="width:240px;height:20px;" align="left" | '''Hugo Raguet'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Applied Mathematics<br />
| style="width:240px;height:20px;" align="left" | Engineer<br />
|-<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.supelec.fr/ Supelec Engineering School] Grad Student<br>and 08' Graduated for [http://www.master-aiv.fr/ Master AIV]<br>'''Interests:''' coming soon...<br />
| style="width:240px;" align="left" |'''Background:'''<br>[http://www.ecp.fr Ecole Centrale des Arts & Manufactures de Paris] Undergrad Student<br>'''Interests:''' Bio, A(p)ero, Psycho. <br>And obviously, [https://2008.igem.org/Image:Ana-Jimenez.jpg the pigeon !]<br />
|-<br />
| style="width:120px;" align="right" rowspan="3"| [[Image:Romain-Rousseau.jpg| 130 px ]]<br />
| style="width:240px;" align="left" | '''[http://xrousseau.com/ Romain Rousseau]'''<br />
|-<br />
| style="width:240px;height:20px;" align="left" | Engineer in Life Sciences<br />
|-<br />
| style="width:240px;" align="left" | '''Background:''' <br>[http://www.paristech.org/ ParisTech] Undergrad Student ([http://www.agroparistech.fr/ Biology and Engineering]) <br> '''Interests:''' Computers, Genetics<br />
<br />
<br />
<br></div>
KP
http://2008.igem.org/File:Photo_158.JPG
File:Photo 158.JPG
2008-09-25T15:23:40Z
<p>KP: </p>
<hr />
<div></div>
KP
http://2008.igem.org/Team:Paris/September_8
Team:Paris/September 8
2008-09-12T14:30:52Z
<p>KP: /* Purification */</p>
<hr />
<div>{{Paris/Calendar_Links|September 7|September 9}}<br />
=Checking our ligases=<br />
Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession.<br />
To do so:<br />
* we will digest a plasmid with only one restriction enzyme (EcoRI).<br />
* then we will try to ligate it back using our ligases.<br />
==Condition tested ==<br />
<br />
[[Image:KR000307.JPG|thumb|Before gel excision <br> well n°4: undigested plasmid (MP101.1) <br> well °6, 7, 8, 9: digested plasmid (D202)]]<br />
[[Image:KR000308.JPG|thumb|After gel excision]]<br />
[[Image:KR000309.JPG|thumb|After purification]]<br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligase'''<br />
|'''incubation time'''<br />
|'''amount of enzyme'''<br />
|-<br />
|L1 (ligase 1)<br />
|2h00<br />
|2 U (unit)<br />
|-<br />
|L1 <br />
|2h00<br />
|400 U <br />
|-<br />
|L1<br />
|O/N<br />
|2 U<br />
|-<br />
|L1<br />
|O/N<br />
|400 U<br />
|-<br />
|L2 (ligase 2)<br />
|2h00<br />
|2 U <br />
|-<br />
|L2<br />
|2h00<br />
|400 U <br />
|-<br />
|L2<br />
|O/N<br />
|2 U<br />
|-<br />
|L2<br />
|O/N<br />
|400 U<br />
|}<br />
<br />
==Digestion==<br />
<br />
Reaction mixture<br />
(carried out four times )<br />
* ADN (MP101.1): 3.8 µL <br />
* H2O: 21.9 µL<br />
* 10X Buffer: 3 µL<br />
* BSA: 0.3 µl<br />
* EcoRI: 1 µL<br />
<br />
==Purification==<br />
<br />
*We purifed our digestion products by gel extraction (Qiagen kit)<br />
*After elution, we obtained [DNA] ~ 25 ng/µL (based on the intensity of the band)<br />
<br />
== Overnight ligation (16°C)==<br />
<br />
* DNA: 4 µL<br />
* 10X Buffer: 2 µL<br />
* Ligase: 1 µL<br />
* H2O: 13 µL</div>
KP
http://2008.igem.org/Team:Paris/September_8
Team:Paris/September 8
2008-09-12T14:30:31Z
<p>KP: /* Digestion */</p>
<hr />
<div>{{Paris/Calendar_Links|September 7|September 9}}<br />
=Checking our ligases=<br />
Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession.<br />
To do so:<br />
* we will digest a plasmid with only one restriction enzyme (EcoRI).<br />
* then we will try to ligate it back using our ligases.<br />
==Condition tested ==<br />
<br />
[[Image:KR000307.JPG|thumb|Before gel excision <br> well n°4: undigested plasmid (MP101.1) <br> well °6, 7, 8, 9: digested plasmid (D202)]]<br />
[[Image:KR000308.JPG|thumb|After gel excision]]<br />
[[Image:KR000309.JPG|thumb|After purification]]<br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligase'''<br />
|'''incubation time'''<br />
|'''amount of enzyme'''<br />
|-<br />
|L1 (ligase 1)<br />
|2h00<br />
|2 U (unit)<br />
|-<br />
|L1 <br />
|2h00<br />
|400 U <br />
|-<br />
|L1<br />
|O/N<br />
|2 U<br />
|-<br />
|L1<br />
|O/N<br />
|400 U<br />
|-<br />
|L2 (ligase 2)<br />
|2h00<br />
|2 U <br />
|-<br />
|L2<br />
|2h00<br />
|400 U <br />
|-<br />
|L2<br />
|O/N<br />
|2 U<br />
|-<br />
|L2<br />
|O/N<br />
|400 U<br />
|}<br />
<br />
==Digestion==<br />
<br />
Reaction mixture<br />
(carried out four times )<br />
* ADN (MP101.1): 3.8 µL <br />
* H2O: 21.9 µL<br />
* 10X Buffer: 3 µL<br />
* BSA: 0.3 µl<br />
* EcoRI: 1 µL<br />
<br />
==Purification==<br />
<br />
*We purifed our digestion products by gel extraction (Qiagen kit)<br />
*After elution, we obtained [DNA] = 25 ng/µL (based on the intensity of the band)<br />
<br />
== Overnight ligation (16°C)==<br />
<br />
* DNA: 4 µL<br />
* 10X Buffer: 2 µL<br />
* Ligase: 1 µL<br />
* H2O: 13 µL</div>
KP
http://2008.igem.org/Team:Paris/September_8
Team:Paris/September 8
2008-09-12T14:30:19Z
<p>KP: /* Digestion */</p>
<hr />
<div>{{Paris/Calendar_Links|September 7|September 9}}<br />
=Checking our ligases=<br />
Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession.<br />
To do so:<br />
* we will digest a plasmid with only one restriction enzyme (EcoRI).<br />
* then we will try to ligate it back using our ligases.<br />
==Condition tested ==<br />
<br />
[[Image:KR000307.JPG|thumb|Before gel excision <br> well n°4: undigested plasmid (MP101.1) <br> well °6, 7, 8, 9: digested plasmid (D202)]]<br />
[[Image:KR000308.JPG|thumb|After gel excision]]<br />
[[Image:KR000309.JPG|thumb|After purification]]<br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligase'''<br />
|'''incubation time'''<br />
|'''amount of enzyme'''<br />
|-<br />
|L1 (ligase 1)<br />
|2h00<br />
|2 U (unit)<br />
|-<br />
|L1 <br />
|2h00<br />
|400 U <br />
|-<br />
|L1<br />
|O/N<br />
|2 U<br />
|-<br />
|L1<br />
|O/N<br />
|400 U<br />
|-<br />
|L2 (ligase 2)<br />
|2h00<br />
|2 U <br />
|-<br />
|L2<br />
|2h00<br />
|400 U <br />
|-<br />
|L2<br />
|O/N<br />
|2 U<br />
|-<br />
|L2<br />
|O/N<br />
|400 U<br />
|}<br />
<br />
==Digestion==<br />
<br />
reaction mixture<br />
(carried out four times )<br />
* ADN (MP101.1): 3.8 µL <br />
* H2O: 21.9 µL<br />
* 10X Buffer: 3 µL<br />
* BSA: 0.3 µl<br />
* EcoRI: 1 µL<br />
<br />
==Purification==<br />
<br />
*We purifed our digestion products by gel extraction (Qiagen kit)<br />
*After elution, we obtained [DNA] = 25 ng/µL (based on the intensity of the band)<br />
<br />
== Overnight ligation (16°C)==<br />
<br />
* DNA: 4 µL<br />
* 10X Buffer: 2 µL<br />
* Ligase: 1 µL<br />
* H2O: 13 µL</div>
KP
http://2008.igem.org/Team:Paris/September_8
Team:Paris/September 8
2008-09-12T14:29:53Z
<p>KP: /* Overnight ligation (16°C) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 7|September 9}}<br />
=Checking our ligases=<br />
Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession.<br />
To do so:<br />
* we will digest a plasmid with only one restriction enzyme (EcoRI).<br />
* then we will try to ligate it back using our ligases.<br />
==Condition tested ==<br />
<br />
[[Image:KR000307.JPG|thumb|Before gel excision <br> well n°4: undigested plasmid (MP101.1) <br> well °6, 7, 8, 9: digested plasmid (D202)]]<br />
[[Image:KR000308.JPG|thumb|After gel excision]]<br />
[[Image:KR000309.JPG|thumb|After purification]]<br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligase'''<br />
|'''incubation time'''<br />
|'''amount of enzyme'''<br />
|-<br />
|L1 (ligase 1)<br />
|2h00<br />
|2 U (unit)<br />
|-<br />
|L1 <br />
|2h00<br />
|400 U <br />
|-<br />
|L1<br />
|O/N<br />
|2 U<br />
|-<br />
|L1<br />
|O/N<br />
|400 U<br />
|-<br />
|L2 (ligase 2)<br />
|2h00<br />
|2 U <br />
|-<br />
|L2<br />
|2h00<br />
|400 U <br />
|-<br />
|L2<br />
|O/N<br />
|2 U<br />
|-<br />
|L2<br />
|O/N<br />
|400 U<br />
|}<br />
<br />
==Digestion==<br />
<br />
reaction mixture<br />
(carried out four times )<br />
* ADN (MP101.1) 3.8 µL <br />
* H2O 21.9 µL<br />
* 10X Buffer 3 µL<br />
* BSA 0.3 µl<br />
* EcoRI 1 µL<br />
<br />
==Purification==<br />
<br />
*We purifed our digestion products by gel extraction (Qiagen kit)<br />
*After elution, we obtained [DNA] = 25 ng/µL (based on the intensity of the band)<br />
<br />
== Overnight ligation (16°C)==<br />
<br />
* DNA: 4 µL<br />
* 10X Buffer: 2 µL<br />
* Ligase: 1 µL<br />
* H2O: 13 µL</div>
KP
http://2008.igem.org/Team:Paris/September_8
Team:Paris/September 8
2008-09-12T14:29:19Z
<p>KP: /* Purification */</p>
<hr />
<div>{{Paris/Calendar_Links|September 7|September 9}}<br />
=Checking our ligases=<br />
Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession.<br />
To do so:<br />
* we will digest a plasmid with only one restriction enzyme (EcoRI).<br />
* then we will try to ligate it back using our ligases.<br />
==Condition tested ==<br />
<br />
[[Image:KR000307.JPG|thumb|Before gel excision <br> well n°4: undigested plasmid (MP101.1) <br> well °6, 7, 8, 9: digested plasmid (D202)]]<br />
[[Image:KR000308.JPG|thumb|After gel excision]]<br />
[[Image:KR000309.JPG|thumb|After purification]]<br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligase'''<br />
|'''incubation time'''<br />
|'''amount of enzyme'''<br />
|-<br />
|L1 (ligase 1)<br />
|2h00<br />
|2 U (unit)<br />
|-<br />
|L1 <br />
|2h00<br />
|400 U <br />
|-<br />
|L1<br />
|O/N<br />
|2 U<br />
|-<br />
|L1<br />
|O/N<br />
|400 U<br />
|-<br />
|L2 (ligase 2)<br />
|2h00<br />
|2 U <br />
|-<br />
|L2<br />
|2h00<br />
|400 U <br />
|-<br />
|L2<br />
|O/N<br />
|2 U<br />
|-<br />
|L2<br />
|O/N<br />
|400 U<br />
|}<br />
<br />
==Digestion==<br />
<br />
reaction mixture<br />
(carried out four times )<br />
* ADN (MP101.1) 3.8 µL <br />
* H2O 21.9 µL<br />
* 10X Buffer 3 µL<br />
* BSA 0.3 µl<br />
* EcoRI 1 µL<br />
<br />
==Purification==<br />
<br />
*We purifed our digestion products by gel extraction (Qiagen kit)<br />
*After elution, we obtained [DNA] = 25 ng/µL (based on the intensity of the band)<br />
<br />
== Overnight ligation (16°C)==<br />
<br />
* DNA 4 µL<br />
* 10X BUFFER 2 µL<br />
* LIGASE 1 µL<br />
* H2O 13 µL</div>
KP
http://2008.igem.org/Team:Paris/September_8
Team:Paris/September 8
2008-09-12T14:28:51Z
<p>KP: /* Purification */</p>
<hr />
<div>{{Paris/Calendar_Links|September 7|September 9}}<br />
=Checking our ligases=<br />
Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession.<br />
To do so:<br />
* we will digest a plasmid with only one restriction enzyme (EcoRI).<br />
* then we will try to ligate it back using our ligases.<br />
==Condition tested ==<br />
<br />
[[Image:KR000307.JPG|thumb|Before gel excision <br> well n°4: undigested plasmid (MP101.1) <br> well °6, 7, 8, 9: digested plasmid (D202)]]<br />
[[Image:KR000308.JPG|thumb|After gel excision]]<br />
[[Image:KR000309.JPG|thumb|After purification]]<br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligase'''<br />
|'''incubation time'''<br />
|'''amount of enzyme'''<br />
|-<br />
|L1 (ligase 1)<br />
|2h00<br />
|2 U (unit)<br />
|-<br />
|L1 <br />
|2h00<br />
|400 U <br />
|-<br />
|L1<br />
|O/N<br />
|2 U<br />
|-<br />
|L1<br />
|O/N<br />
|400 U<br />
|-<br />
|L2 (ligase 2)<br />
|2h00<br />
|2 U <br />
|-<br />
|L2<br />
|2h00<br />
|400 U <br />
|-<br />
|L2<br />
|O/N<br />
|2 U<br />
|-<br />
|L2<br />
|O/N<br />
|400 U<br />
|}<br />
<br />
==Digestion==<br />
<br />
reaction mixture<br />
(carried out four times )<br />
* ADN (MP101.1) 3.8 µL <br />
* H2O 21.9 µL<br />
* 10X Buffer 3 µL<br />
* BSA 0.3 µl<br />
* EcoRI 1 µL<br />
<br />
==Purification==<br />
<br />
*We purifed our digestion products by gel extraction (Qiagen kit)<br />
*After elution, we obtained [DNA] = 25 ng/µL<br />
<br />
== Overnight ligation (16°C)==<br />
<br />
* DNA 4 µL<br />
* 10X BUFFER 2 µL<br />
* LIGASE 1 µL<br />
* H2O 13 µL</div>
KP
http://2008.igem.org/Team:Paris/September_8
Team:Paris/September 8
2008-09-12T14:28:40Z
<p>KP: /* Purification */</p>
<hr />
<div>{{Paris/Calendar_Links|September 7|September 9}}<br />
=Checking our ligases=<br />
Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession.<br />
To do so:<br />
* we will digest a plasmid with only one restriction enzyme (EcoRI).<br />
* then we will try to ligate it back using our ligases.<br />
==Condition tested ==<br />
<br />
[[Image:KR000307.JPG|thumb|Before gel excision <br> well n°4: undigested plasmid (MP101.1) <br> well °6, 7, 8, 9: digested plasmid (D202)]]<br />
[[Image:KR000308.JPG|thumb|After gel excision]]<br />
[[Image:KR000309.JPG|thumb|After purification]]<br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligase'''<br />
|'''incubation time'''<br />
|'''amount of enzyme'''<br />
|-<br />
|L1 (ligase 1)<br />
|2h00<br />
|2 U (unit)<br />
|-<br />
|L1 <br />
|2h00<br />
|400 U <br />
|-<br />
|L1<br />
|O/N<br />
|2 U<br />
|-<br />
|L1<br />
|O/N<br />
|400 U<br />
|-<br />
|L2 (ligase 2)<br />
|2h00<br />
|2 U <br />
|-<br />
|L2<br />
|2h00<br />
|400 U <br />
|-<br />
|L2<br />
|O/N<br />
|2 U<br />
|-<br />
|L2<br />
|O/N<br />
|400 U<br />
|}<br />
<br />
==Digestion==<br />
<br />
reaction mixture<br />
(carried out four times )<br />
* ADN (MP101.1) 3.8 µL <br />
* H2O 21.9 µL<br />
* 10X Buffer 3 µL<br />
* BSA 0.3 µl<br />
* EcoRI 1 µL<br />
<br />
==Purification==<br />
<br />
*We purifed our digestion products by gel extraction (Qiagen kit)<br />
*After elution, we obtained [DNA] = 24 ng/µL<br />
<br />
== Overnight ligation (16°C)==<br />
<br />
* DNA 4 µL<br />
* 10X BUFFER 2 µL<br />
* LIGASE 1 µL<br />
* H2O 13 µL</div>
KP
http://2008.igem.org/Team:Paris/September_9
Team:Paris/September 9
2008-09-12T14:26:48Z
<p>KP: /* Transformation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 8|September 10}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Ligation (2 hours at 16°C)==<br />
<br />
*4 µL DNA<br />
*2 µL 10X buffer 2<br />
*1 µL T4 DNA ligase<br />
*13 µL water<br />
<br />
==Transformation==<br />
<br />
*Strain used: Chemically competent E. coli DH5 alpha cells<br />
*100 µL of cells<br />
*5 µL of ligation products<br />
*30 min on ice<br />
*heat shock: 45 s at 37°C<br />
*2 min on ice<br />
*900 µL of SOC<br />
*1 hour at 37°C<br />
*plating (1 µL or 100 µL) on LB plates containing ampicilline</div>
KP
http://2008.igem.org/Team:Paris/September_10
Team:Paris/September 10
2008-09-12T14:26:11Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 9|September 11}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation transformants==<br />
<br />
*Several colonies for the pUC19 transformation control<br />
*No colonies for the ligations<br />
<br />
==Analysis of the ligation products by electrophoresis==<br />
<br />
[[Image:KR000310.JPG|thumb]]<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|14<br />
|15<br />
|-<br />
|'''sample'''<br />
|rowspan="5"|1 kb <br> DNA ladder<br />
|rowspan="5"|undigested DNA <br> MP101.1<br />
|colspan="12"|'''ligation products'''<br />
|rowspan="5"|100 bp <br> DNA ladder<br />
|-<br />
|'''temperature of ligation'''<br />
|colspan="8"|16°C<br />
|colspan="4"|4°C<br />
|-<br />
|'''ligase n°'''<br />
|colspan="4"|L1<br />
|colspan="4"|L2<br />
|colspan="2"|L1<br />
|colspan="2"|L2<br />
|-<br />
|'''duration of ligation'''<br />
|colspan="2"|2 h<br />
|colspan="2"|O/N<br />
|colspan="2"|2 h<br />
|colspan="6"|O/N<br />
|-<br />
|'''amount of ligase (units)'''<br />
|400 U<br />
|2 U<br />
|400 U<br />
|2 U<br />
|400 U<br />
|2 U<br />
|400 U<br />
|2 U<br />
|400 U<br />
|2 U<br />
|400 U<br />
|2 U<br />
|-<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T14:22:28Z
<p>KP: /* Purification */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
<br><br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
<br><br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
'''3 ligases tested'''<br />
*ligase '''1''': our 250 µL (400 000 U/mL) tube<br />
*ligase '''2''': our 50 µL (400 000 U/mL) tube<br />
*ligase '''3''': 2nd floor 50 µL (400 000 U/mL) tube<br />
The ligase 1 and 2 have been used with our own buffer tube, whereas the ligase 3 has been used with the buffer tube from the 2nd floor lab.<br />
<br><br />
<br>'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br><br />
<br>'''Reaction mixture for unpurified digestion products'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T14:22:15Z
<p>KP: /* Purification */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
<br><br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
'''3 ligases tested'''<br />
*ligase '''1''': our 250 µL (400 000 U/mL) tube<br />
*ligase '''2''': our 50 µL (400 000 U/mL) tube<br />
*ligase '''3''': 2nd floor 50 µL (400 000 U/mL) tube<br />
The ligase 1 and 2 have been used with our own buffer tube, whereas the ligase 3 has been used with the buffer tube from the 2nd floor lab.<br />
<br><br />
<br>'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br><br />
<br>'''Reaction mixture for unpurified digestion products'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T14:20:07Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
'''3 ligases tested'''<br />
*ligase '''1''': our 250 µL (400 000 U/mL) tube<br />
*ligase '''2''': our 50 µL (400 000 U/mL) tube<br />
*ligase '''3''': 2nd floor 50 µL (400 000 U/mL) tube<br />
The ligase 1 and 2 have been used with our own buffer tube, whereas the ligase 3 has been used with the buffer tube from the 2nd floor lab.<br />
<br><br />
<br>'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br><br />
<br>'''Reaction mixture for unpurified digestion products'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T14:19:23Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
'''3 ligases tested'''<br />
*ligase '''1''': our 250 µL (400 000 U/mL) tube<br />
*ligase '''2''': our 50 µL (400 000 U/mL) tube<br />
*ligase '''3''': 2nd floor 50 µL (400 000 U/mL) tube<br />
The ligase 1 and 2 were used with our buffer tube, whereas the ligase 3 was used with the buffer tube from the 2nd floor lab.<br />
<br><br />
<br>'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br><br />
<br>'''Reaction mixture for unpurified digestion products'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T14:18:28Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
The ligase 1 and 2 were used with our buffer tube, whereas the ligase 3 was used with the buffer tube from the 2nd floor lab.<br />
<br><br />
<br>'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br><br />
<br>'''Reaction mixture for unpurified digestion product'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
<br />
<br>All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T14:17:58Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
The ligase 1 and 2 were used with our buffer tube, whereas the ligase 3 was used with the buffer tube from the 2nd floor lab.<br />
<br><br />
<br>'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br><br />
<br>'''Reaction mixture for unpurified digestion product'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T14:17:41Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
The ligase 1 and 2 were used with our buffer tube, whereas the ligase 3 was used with the buffer tube from the 2nd floor lab.<br />
<br><br />
'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br><br />
'''Reaction mixture for unpurified digestion product'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:16:25Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
1% agarose gel<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by '''EcoRI''']]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by '''BspHI''']]<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
<br>No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid <br> (MP101)<br />
|rowspan="3"|circular <br> plasmid <br> (MP101) <br />
|rowspan="3"|digested <br> plasmid <br> (D202) <br />
|rowspan="3"|digested <br> plasmid <br> (D202) <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification of <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
<br><br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
<br>Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid <br> (MP101)<br />
|rowspan="3"|circular <br> plasmid <br> (MP101) <br />
|rowspan="3"|digested <br> plasmid <br> (D203)<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification of <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:14:19Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
1% agarose gel<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by '''EcoRI''']]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by '''BspHI''']]<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
<br>No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid <br> (MP101)<br />
|rowspan="3"|circular <br> plasmid <br> (MP101) <br />
|rowspan="3"|digested <br> plasmid <br> (D202) <br />
|rowspan="3"|digested <br> plasmid <br> (D202) <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
<br><br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
<br>Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid <br> (MP101)<br />
|rowspan="3"|circular <br> plasmid <br> (MP101) <br />
|rowspan="3"|digested <br> plasmid <br> (D203)<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:13:43Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
1% agarose gel<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
<br>No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid <br> (MP101)<br />
|rowspan="3"|circular <br> plasmid <br> (MP101) <br />
|rowspan="3"|digested <br> plasmid <br> (D202) <br />
|rowspan="3"|digested <br> plasmid <br> (D202) <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
<br><br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
<br>Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid <br> (MP101)<br />
|rowspan="3"|circular <br> plasmid <br> (MP101) <br />
|rowspan="3"|digested <br> plasmid <br> (D203)<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:11:48Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
1% agarose gel<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
<br>No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid <br> (MP101)<br />
|rowspan="3"|circular <br> plasmid <br> (MP101) <br />
|rowspan="3"|digested <br> plasmid <br> (D202) <br />
|rowspan="3"|digested <br> plasmid <br> (D202) <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
<br>Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid <br> (MP101)<br />
|rowspan="3"|circular <br> plasmid <br> (MP101) <br />
|rowspan="3"|digested <br> plasmid <br> (D203)<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:11:01Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
1% agarose gel<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
<br>No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid (MP101)<br />
|rowspan="3"|circular <br> plasmid (MP101) <br />
|rowspan="3"|digested <br> plasmid (D202) <br />
|rowspan="3"|digested <br> plasmid (D202) <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
<br>Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|circular <br> plasmid (MP101)<br />
|rowspan="3"|circular <br> plasmid (MP101) <br />
|rowspan="3"|digested <br> plasmid (D203)<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:07:42Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
1% agarose gel<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
<br>No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D202 <br />
|rowspan="3"|D202 <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
<br>Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D203<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:07:31Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
1% agarose gel<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D202 <br />
|rowspan="3"|D202 <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
<br>Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D203<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:07:11Z
<p>KP: /* Analysis of the ligation products by electrophosesis */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
1% agarose gel<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D202 <br />
|rowspan="3"|D202 <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D203<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:06:43Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D202 <br />
|rowspan="3"|D202 <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}<br />
<br />
'''Analysis of the ligation of the plasmid digested by BspHI'''<br />
Heat inactivation of the ligase (20 min at 65°C)<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D203<br />
|'''8'''<br />
|'''9'''<br />
|'''10'''<br />
|'''11'''<br />
|'''12'''<br />
|'''13'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,3 µL<br />
|0,5 µL<br />
|4 µL<br />
|colspan="3"|15 µL<br />
|colspan="3"|10 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:02:31Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D202 <br />
|rowspan="3"|D202 <br />
|'''1'''<br />
|'''2'''<br />
|'''3'''<br />
|'''4'''<br />
|'''5'''<br />
|'''6'''<br />
|'''7'''<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:01:51Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3"|MP101<br />
|rowspan="3"|MP101 <br />
|rowspan="3"|D202 <br />
|rowspan="3"|D202 <br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T14:01:00Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3|MP101<br />
|rowspan="3|MP101 <br />
|rowspan="3"|D202 <br />
|rowspan="3"|D202 <br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T13:59:07Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
No heat inactivation of the ligase<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|rowspan="3"|1 kb ladder<br />
|rowspan="3|MP101<br />
|rowspan="3|MP101 <br />
|rowspan="3"|D202 <br />
|rowspan="3"|D202 <br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|rowspan="3"|100 bp ladder<br />
|-<br />
|'''purification <br> digestion products'''<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|-<br />
|'''amount loaded'''<br />
|10 µL<br />
|0,5 µL<br />
|1 µL<br />
|2 µL<br />
|3 µL<br />
|colspan="6"|10 µL<br />
|3 µL<br />
|10 µL<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T13:51:43Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 0,5 µL<br />
|MP101 <br> 1 µL<br />
|D202 <br> 2 µL<br />
|D202 <br> 3 µL<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|100 bp ladder<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T13:49:10Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 0,5 µL<br />
|MP101 <br> 1 µL<br />
|D202 <br> 2 µL<br />
|D202 <br> 3 µL<br />
|Ligation 1<br />
|Ligation 2<br />
|Ligation 3<br />
|Ligation 4<br />
|Ligation 5<br />
|Ligation 6<br />
|Ligation 7<br />
|100 bp ladder<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T13:48:08Z
<p>KP: </p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
[[Image:Ligation-D203.JPG|thumb|Ligation of the plasmid digested by BspHI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 0,5 µL<br />
|MP101 <br> 1 µL<br />
|D202 <br> 2 µL<br />
|D202 <br> 3 µL<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|100 bp ladder<br />
|}</div>
KP
http://2008.igem.org/File:Ligation-D203.JPG
File:Ligation-D203.JPG
2008-09-12T13:47:08Z
<p>KP: </p>
<hr />
<div></div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T13:35:32Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
The ligase 1 and 2 were used with our buffer tube, whereas the ligase 3 was used with the buffer tube from the 2nd floor lab.<br />
'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br>'''Reaction mixture for unpurified digestion product'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T13:33:15Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br>'''Reaction mixture for unpurified digestion product'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
All the ligation were carried out '''overnight''' at '''4°C'''. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T13:32:41Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
<br>'''Reaction mixture for unpurified digestion product'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
All the ligation were carried out overnight at 4°C. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T13:32:27Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
'''Reaction mixture for unpurified digestion product'''<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
All the ligation were carried out overnight at 4°C. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T13:31:24Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
'''Reaction mixture'''<br />
*8 µL of purified digestion products<br />
*2 µL of T4 DNA ligase 10X buffer<br />
*9 µL of water<br />
*1 µL of T4 DNA ligase<br />
For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
*8 µL of unpurified<br />
*1 µL of ATP 10 mM (1mM final concentration)<br />
*1 µL of T4 DNA ligase<br />
All the ligation were carried out overnight at 4°C. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_11
Team:Paris/September 11
2008-09-12T13:24:01Z
<p>KP: /* Ligation */</p>
<hr />
<div>{{Paris/Calendar_Links|September 10|September 12}}<br />
<br />
=Checking our ligases=<br />
<br />
Because we couldn't be sure of the results of yesterday experiment, we decided to carry it out one more time.<br />
<br />
==Digestion==<br />
<br />
[[Image:Before-excision.JPG|thumb|'''Before excision''']]<br />
[[Image:After-excision.JPG|thumb|'''After ecxision''']]<br />
<br />
DNA used for digestion: MP101<br />
<br> 2 digestion were carried out:<br />
*one with EcoRI (D202), that cuts the plasmid only one time <br />
*the other with BspHI (D203), that cuts the plasmid three times<br />
<br />
'''Reaction mixture'''<br />
*10 µL of DNA<br />
*6µL of 10X buffer 2<br />
*0,6 µL of 100X BSA<br />
*2 µL of enzyme (EcoRI or BspHI)<br />
*41,4 µL of water<br />
2h30 at 37°C and then 20 min at 65°C<br />
<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 1 µL<br />
| <br />
|D202 <br> 10 µL<br />
|D202 <br> 10 µL<br />
| <br />
|D203 <br> 3 µL<br />
|100 bp ladder<br />
|-<br />
|'''expected size'''<br />
|<br />
|<br />
|<br />
|colspan="2"|2984 bp<br />
|<br />
|1870 bp <br> 1008 bp <br> 105 bp<br />
|<br />
|-<br />
|'''measured size'''<br />
|<br />
|<br />
|<br />
|'''3 kb'''<br />
|'''3 kb'''<br />
|<br />
|'''1,9 kb''' <br> '''1 kb'''<br />
|<br />
|}<br />
<br />
==Purification==<br />
<br />
[[Image:After-purification.JPG|thumb|'''After purification''' <br> well n°2: D202 purified by gel <br> well n°3: D202 purified by column <br> well n°4: D203 purified by column <br> well n°5: MP101]]<br />
<br />
D202 (EcoRI digestion) was purified in 2 ways:<br />
*by gel extraction<br />
*or by column<br />
D203 (BspHI digestion) was purified:<br />
*by column<br />
Elution in 30 µL of EB buffer<br />
<br />
==Ligation==<br />
<br />
3 ligases tested<br />
*ligase 1: our 250 µL (400 000 U/mL) tube<br />
*ligase 2: our 50 µL (400 000 U/mL) tube<br />
*ligase 3: 2nd floor 50 µL (400 000 U/mL) tube<br />
'''Reaction mixture'''<br />
<br />
For the samples for which the digestion products have not been purified, 1 µL of ligase and 1 µL of ATP (1 mM final concentration) have been added directly in the digestion products (in the digestion buffer 2).<br />
All the ligation were carried out overnight at 4°C. <br />
<br />
{|border="1" style="text-align: center"<br />
|'''ligation'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''digested DNA used'''<br />
|colspan="7"|D202 (EcoRI digestion)<br />
|colspan="6"|D203 (BspHI digestion)<br />
|-<br />
|'''type of purification <br> of the digestion products<br />
|colspan="3"|gel extraction<br />
|colspan="3"|column<br />
|no <br> purification<br />
|colspan="3"|column<br />
|colspan="3"|no <br> purification<br />
|-<br />
|'''ligase used'''<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L1<br />
|L2<br />
|L3<br />
|L1<br />
|L2<br />
|L3<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T13:22:09Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 0,5 µL<br />
|MP101 <br> 1 µL<br />
|D202 <br> 2 µL<br />
|D202 <br> 3 µL<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|100 bp ladder<br />
|}</div>
KP
http://2008.igem.org/Team:Paris/September_12
Team:Paris/September 12
2008-09-12T13:21:49Z
<p>KP: /* Checking our ligases (following) */</p>
<hr />
<div>{{Paris/Calendar_Links|September 11|September 13}}<br />
<br />
=Checking our ligases (following)=<br />
<br />
==Analysis of the ligation products by electrophosesis==<br />
<br />
[[Image:Ligation-D202.JPG|thumb|Ligation of the plasmid digested by EcoRI]]<br />
<br />
1% agarose gel<br />
<br />
'''Analysis of the ligation of the plasmid digested by EcoRI'''<br />
{|border="1" style="text-align: center"<br />
|'''well n°'''<br />
|1<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|9<br />
|10<br />
|11<br />
|12<br />
|13<br />
|-<br />
|'''sample'''<br />
|1 kb ladder<br />
|MP101 <br> 0,5 µL<br />
|MP101 <br> 1 µL<br />
|D202 <br> 2 µL<br />
|D202 <br> 3 µL<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|<br />
|}</div>
KP