Search results

From 2008.igem.org

Create the page "Standard Media" on this wiki!

  • Team:TUDelft/Protocols
    * Grow a 5ml overnight culture of cells in LB media. * In the morning, dilute this culture back into 25-50ml of fresh LB media in a 200ml conical flask. You should aim to dilute the overnight culture by
    19 KB (3,279 words) - 23:04, 29 October 2008
  • Team:Harvard/GenProtocols
    #Remove media and wash with 0.33 volumes (330μL) of 1M sorbitol #Grow 5mL culture of bacteria in LB media overnight, 37° C , shaking
    13 KB (2,102 words) - 05:38, 29 October 2008
  • Team:Heidelberg/Notebook/Killing II/11thweek
    ... -> 200 µl Referencepromotercells + 200 µl Receivercells + 600 µl TB media ... -> 200 µl Referencepromotercells + 40 µl Receivercells + 760 µl TB media
    36 KB (4,963 words) - 21:50, 28 October 2008
  • Team:iHKU/protocol
    ... were collected by centrifugation, <em>ca</em>. 900 μl of supernatant media was discarded, and then the resuspended cells were plated onto LB agar con A 50 mL culture of EColi. was grown in 2% LB media overnight (16 hours) in 37’C shaker. A fresh 50 mL LB culture was inocul
    63 KB (6,466 words) - 19:25, 29 October 2008
  • Team:KULeuven/Data/GFP
    ==== Media ==== The media used were Luria-Bertani (LB) medium or ABT minimal medium. LB medium was us
    11 KB (1,747 words) - 15:41, 19 October 2008
  • Team:University of Washington/Project
    ...ry that provided a PoPS output when these three conditions are met. Using standard BioBrick assembly methods, we placed the gene for LuxR under the control of ...design of projects like ours easier using information from the Registry of Standard Biological Parts (see SeToB tab).
    23 KB (3,437 words) - 04:40, 30 October 2008
  • Team:Bologna/Protocols
    ...istilled water-EOH 95%) into the spot that corresponds to the Biobrick™-standard part that you want. # Put 5 ml of LB media in a falcon tube.
    13 KB (2,058 words) - 13:45, 28 October 2008
  • Team:NTU-Singapore/Wetlab/Materials and Equipment
    ==Bacteria strains and media== ...ht growth of cells were Luria-Bertani broth (LB). Cells were diluted in M9 media to an OD600 of 1 before ready to be read under Fluorescence Microplate Read
    6 KB (869 words) - 05:47, 27 October 2008
  • Team:Michigan/Notebook/TransformationProtocol
    == <font color=royalblue size=5> Transformation Standard Protocol </font> == 7. Add 900 &mu;L pre-warmed LB media & allow to recover on spinning wheel in incubator (37 degrees Celsius) for
    2 KB (239 words) - 03:06, 30 October 2008
  • Team:Cambridge/Signaling
    Bacillus strain 1A1 (derivative of standard strain 168) * deficient in tryptophan, have to add to media
    7 KB (1,055 words) - 14:52, 28 October 2008
  • Team:Cambridge/Signalling
    Bacillus strain 1A1 (derivative of standard strain 168) * deficient in tryptophan, have to add to media
    6 KB (848 words) - 04:26, 30 October 2008
  • Team:ETH Zurich/Project/Background
    ...ticellularity or a nucleus can live a happy life with even less genes. The standard laboratory bacterium Escherichia coli is happy with just 4,467 genes (5, 6) ...s biosynthetic pathways that are not needed for growth in standard glucose media and that therefore only add to the costs of protein synthesis without incre
    9 KB (1,351 words) - 09:58, 30 October 2008
  • Everything you ever wanted to know about AarI
    ...lves the biggest problem faced when trying to do multipart ligations using standard restriction enzymes, the self ligation of a part, blocking it's incorporati ...serts). While parts can be made with any 4 base overhang (end), we chose a standard set, termed A, B, C, and D. This allows parts to be traded between research
    6 KB (1,025 words) - 04:20, 29 October 2008
  • Team:Bologna/Notebook
    ...rk: week by week you can find all the procedures, links to the registry of standard parts and protocols. The chronological structure of this section, organized ... to test and set up the new [http://partsregistry.org/Measurement Biobrick Standard Measurement Protocol]
    15 KB (2,315 words) - 01:13, 30 October 2008
  • Team:Rice University/RESULTS
    ...nd the consumption of ''p''-coumaric acid, which will only be added to the media for the 4CL::STS-integrated yeast; the 4CL::STS+TAL will produce resveratro ...b|700px|'''Figure C1. Example HPLC chromatogram of a ''trans''-resveratrol standard.''']]
    7 KB (962 words) - 05:22, 30 October 2008
  • Team:Johns Hopkins/Notebook/GROUP 3: Short two way stops
    [[Media:10-4-08 digest of 10;11;13.jpg]] [[Media:10-04-08 PCR verification.jpg]]
    7 KB (1,012 words) - 01:06, 29 October 2008
  • Team:Hawaii/Project/Part B MaterialsMethodsResults
    ...pilA'' and ''slr2016'' secretion signal sequences were syntheized with the standard Biobrick sites. Oligonucleotide fragments of each were hybridized with its Following standard PCR protocol, [http://www.partsregistry.org/Part:BBa_E0040 GFP] was convert
    13 KB (1,867 words) - 03:04, 30 October 2008
  • /Project/Vitamins
    ...nating in a double stop (TAATAA) sequence, as regulated by the Registry of Standard Biological Parts. ...e'' given known quantities of folate present in the growth media. Once the standard curve has been established, then experimental growth levels, as quantified
    10 KB (1,460 words) - 17:32, 22 August 2008
  • Team:Bologna/Software
    ...h segmented bacteria the software computes the size in pixel, the mean and standard deviation of 
intensity florescence. The use of the software is easy and ...of pixels) and obtain informations about their area, fluorescence mean and standard deviation. Fluorescence is read from R channel for RFP, G for GFP and B for
    6 KB (826 words) - 11:10, 27 April 2016
  • Team:Heidelberg/Notebook/Killing I/Notebook/week8
    * preparation of in vitro packaging media === Cloning oriT in standard plasmid ===
    27 KB (3,801 words) - 11:47, 29 October 2008

View (previous 20 | next 20) (20 | 50 | 100 | 250 | 500)