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Create the page "Standard Media" on this wiki!

• Main Page
  ...oree? See the <a class="pink" href="https://2008.igem.org/Jamboree/Media">Media</a> page for pictures and videos. Post your own links to let us know where **[[Jamboree/Media | Media ]]<small>(pictures & fun videos!)</small>
  10 KB (1,324 words) - 20:30, 5 May 2016
• Team:Illinois/Antibody GPCR Fusion
  ...of the FUS1-Fluo.Prot. fusion into the yeast genome, which seems to be the standard for this type of thing. *Preparation of Yeast Media [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/m
  21 KB (3,227 words) - 02:19, 24 October 2008
• Team:Hawaii/Project/Part B
  ...A'' and ''slr2016'' secretion signal sequences will be syntheized with the standard Biobrick sites. Oligonucleotide fragments of each will be hybridized with i ...efficacy of the signal peptides in transporting GFP into the extracellular media can be measured using a spectrofluorometer.
  11 KB (1,558 words) - 02:25, 29 October 2008
• Team:University of Chicago/Notebook/Norayucel
  7. Added 250microliters SOC media (prepared at 1:30)<br> ...0microliters for pGreen, 1mL for E0240 (scaled up to accommodate for extra media dilution)
  11 KB (1,571 words) - 16:42, 28 September 2008
• Team:EPF-Lausanne/Notebook
  ===Media and buffers=== ..., animals or the environment, other than the ones which are encountered in standard laboratory work with ''E.coli''. Therefore, we estimate the risk to researc
  16 KB (2,322 words) - 23:27, 29 October 2008
• Team:Heidelberg/Human Practice/Open Day
  ...translated the text in english, the original german version can be found [[Media:IGEM-Veranstaltung_Heidelberg.pdf|'''here''']]). ...en_Day_english.ppt.html here] (rapdishare download file) and as pdf-file [[Media:Open_Day_english.pdf| here]]. <br/>
  60 KB (9,576 words) - 15:58, 29 October 2008
• Team:UCSF/Materials and Methods
  ...gal2::NatR, allowing graded activation of galactose inducible promoters ([[Media:Hawkins and Smolke.pdf]]). ...ere grown to early log phase (OD600=0.1-0.3) in complete synthetic dropout media and then treated with 1 μM α-factor (Zymo Research) to activate the pathw
  3 KB (499 words) - 03:27, 30 October 2008
• Team:Alberta NINT/Project
  [[Media:NINT_PlasmidDesignAndConstruction.pdf|Plasmid Design and Construction]] ...ion in the absence of any input. The y-axis is shown in Miller units (the standard for such assays).
  21 KB (3,119 words) - 16:44, 30 March 2009
• Team:Hawaii/Member Training
  ## Read [[Image:Idempotent Vector Design for Standard Assembly of Biobricks.pdf|Biobrick Technical Manual]], don't worry about un ## [http://igem.blip.tv/file/254379 Drew Endy: What is a Standard Biological Part] 2 minute 31 seconds
  2 KB (309 words) - 12:16, 31 May 2008
• Team:UC Berkeley/Protocols
  # Plate using the sterilized glass-bead method or with standard spreaders. # Plate using the sterilized glass-bead method or with standard spreaders.
  17 KB (2,857 words) - 22:03, 27 October 2008
• Jamboree/Compete
  ...mboree/Compete#Requests_for_variance">Submit any requests for non-BioBrick standard methods</a> : <span class="deadline">October 8</span></li> ...xpected to submit their parts to the [http://partsregistry.org Registry of Standard Biological Parts]. In addition to submitting your parts, you must also dec
  13 KB (1,920 words) - 06:14, 22 April 2016
• Team:Waterloo/Notebook
  ...plication written in-house for administration of BioBricks. It understands standard assembly and can gather information from the Parts Registry. BioMortar will ...hat it can construct sequences for composite parts automatically. Although Standard Assembly makes it easy to ligate parts, it is fairly easy to reverse the en
  3 KB (456 words) - 04:05, 30 October 2008
• SOC
  #Follow directions to make 1 liter of [[SOB]] media [[Category:Material]] [[Category:Standard Media]]
  546 B (73 words) - 02:24, 30 May 2008
• Team:Waterloo/Project
  ...nucleases during uninduced conditions. Cultures will initially be grown on media containing IPTG. Expression of the unstable cI repressor (C0051) will be dr ...me degradation construct will be integrated into the genome via a proposed standard integration vector.
  11 KB (1,711 words) - 04:00, 30 October 2008
• Team:Groningen/Notebook
  ...e [http://partsregistry.org/Assembly:Standard_assembly <font color=#dd0000>Standard Assembly from the parts registry</font color>] site. When we had to assembl '''Media'''
  4 KB (643 words) - 12:10, 16 September 2008
• Alberta NINT/23 July 2008
  showed sufficient cell growth after two days at 37 C. Prepared media (LB+antibiotics+IPTG+glucose/arabinose) and inoculated 1 ml of media with 20 ul of cell cultures
  968 B (108 words) - 23:02, 11 October 2008
• Team:Davidson-Missouri Western
  ...rts. We also produced a graphical interface for exploring the Registry of Standard Biological Parts called Viz-A-Brick, and other web-based tools to improve t i'''GEM 2008 powerpoint presentation''' [[Media:MWSU_Davidson_iGEM08.PPT]]
  4 KB (583 words) - 03:55, 30 October 2008
• Team:University of Washington/Measurement Kit
  ... After 16 hours, each overnight culture was diluted 100 fold into fresh M9 media with kanamycin, and grown for 3 hours. After three hours, two 200uL samples
  4 KB (577 words) - 04:09, 30 October 2008
• Team:Peking University/Project
  ...s yeast codon optimized kindly offered by Prof. Youri I Pavlov. A set of [[Media:Linkers.pdf|12 linkers]] were synthesized to test the ability to maintain t ...flanking the 3’ of the promoter. Other constructs were accomplished with standard protocols.
  18 KB (2,815 words) - 05:26, 30 October 2008
• Team:Heidelberg/Notebook/Killing II/5thweek
  *Retransformation of ligation into TOP 10 -> standard protocoll *Preparation of M9 media
  27 KB (3,683 words) - 21:27, 28 October 2008